The Eukaryotic RNA Exosome [E-Book] : Methods and Protocols / edited by John LaCava, Štěpánka Vaňáčová.
This volume provides a cross-section of RNA exosome research protocols, applied to a diversity of model organisms. Chapters guide readers through methods that e.g. delineate eukaryotic exosomes' origins in prokaryotes, probe its RNA substrates, adapter complexes, and macromolecular interaction...
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Full text |
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Personal Name(s): | LaCava, John, editor |
Vaňáčová, Štěpánka, editor | |
Edition: |
1st edition 2020. |
Imprint: |
New York, NY :
Human Press,
2020
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Physical Description: |
XIII, 517 pages 79 illustrations, 52 illustrations in color (online resource) |
Note: |
englisch |
ISBN: |
9781493998227 |
DOI: |
10.1007/978-1-4939-9822-7 |
Series Title: |
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Methods in Molecular Biology ;
2062 |
Subject (ZB): | |
Subject (LOC): | |
Classification: |
- The RNA Exosome and Human Disease
- The Bacterial Counterparts of the Eukaryotic Exosome: An Evolutionary Perspective
- In vitro Characterization of the Prokaryotic Counterparts of the Exosome Complex
- Enzymatic Analysis of Reconstituted Archaeal Exosomes
- Protocols for Northern Analysis of Exosome Substrates and Other Non-coding RNAs
- Mapping Exosome-substrate Interactions in vivo by UV-crosslinking
- Global Identification of Human Exosome Substrates using RNA Interference and RNA-sequencing
- High-resolution Mapping of 3' Extremities of RNA Exosome Substrates by 3' RACE-seq
- Determining mRNA Stability by Metabolic RNA Labeling and Chemical Nucleoside Conversion.-Thiouridine-to-Cytidine Conversion Sequencing (TUC-seq) to Measure mRNA Transcription and Degradation Rates
- RNA Exosomes and their Co-factors
- Purification of Endogenous Tagged TRAMP4/5 and Exosome Complexes from Yeast and in vitro Polyadenylation-Exosome Activation Assays
- Comparative poly(A)+ RNA Interactome Capture of RNA Surveillance Mutants
- Purification and in vitro Analysis of the Exosome Cofactors Nrd1-Nab3 and Trf4-Air2
- Affinity Proteomic Analysis of the Human Exosome and its Cofactor Complexes
- In vitro Characterization of the Activity of the Mammalian RNA exosome on mRNAs in Ribosomal Translation Complexes
- Native Mass Spectrometry Analysis of Affinity-captured Endogenous Yeast RNA Exosome Complexes
- Chemical Cross-Linking and Mass Spectrometric Analysis of the Endogenous Yeast Exosome Complexes
- Cryo-Electron Microscopy of Endogenous Yeast Exosomes
- Strategies for Generating RNA Exosome Complexes from Recombinant Expression Hosts
- Reconstitution of S. cerevisiae RNA Exosome Complexes Using Recombinantly Expressed Proteins
- Reconstitution of the Schizosaccharomyces pombe RNA Exosome
- Reconstitution of the Human Nuclear RNA Exosome
- Purification and Reconstitution of the S. cerevisiae TRAMP and Ski Complexes for Biochemical and Structural Studies. .