Inositol Phosphates [E-Book] : Methods and Protocols / edited by Gregory J. Miller.
Miller, Gregory J., (editor)
1st edition 2020.
New York, NY : Human Press, 2020
X, 185 pages 35 illustrations, 15 illustrations in color (online resource)
Methods in Molecular Biology ; 2091
Full Text
Table of Contents:
  • A Short Historical Perspective of Methods in Inositol Phosphate Research
  • Quantitation of Inositol Phosphates by HPLC-ESI-MS
  • Isolation of Inositol Hexakisphosphate from Soils by Alkaline Extraction and Hypobromite Oxidation
  • Detection of Inositol Phosphates by Split PH Domains
  • Dictyostelium discoideum as a Model to Study Inositol Pyrophosphate and Inorganic Polyphosphate
  • Characterising Enzymes of the Diphosphoinositol Polyphosphate Phosphohydrolase (DIPP) Family
  • Metabolic Labeling of Inositol Phosphates and Phosphatidylinositols in Yeast and Mammalian Cells
  • Back-pyrophosphorylation Assay to Detect in vivo InsP7-dependent Protein Pyrophosphorylation in Mammalian Cells
  • Analyses of Ligand Binding to IP3 receptors using Fluorescence Polarization
  • Investigating the InsP3 Receptor in Living Cells by Caged InsP3
  • Optical Measurement of Synaptic Vesicle Fusion and its Inhibition by Inositol Pyrophosphate in Primary Cultured Hippocampal Neurons
  • Competitive Fluorescent Ligand Assay for Inositol 1,4,5-Trisphosphate
  • Studying the Effects of Inositol Pyrophosphates in an In Vitro Vesicle-vesicle Fusion Assay
  • Engineering Protease Resistant and Highly Active Phytases
  • Direct Cloning Method for Expression of Recombinant Proteins with an Inositol Hexakisphosphate Inducible Self-cleaving Tag
  • A Short Historical Perspective of Methods in Inositol Phosphate Research. .