Animal Cell Biotechnology [E-Book] : Methods and Protocols / edited by Ralf Pörtner.
Pörtner, Ralf, (editor)
4th edition 2020.
New York, NY : Humana Press, 2020
XIV, 399 pages 127 illustrations, 80 illustrations in color (online resource)
Methods in Molecular Biology ; 2095
Full Text
Table of Contents:
  • Near-Physiological Cell-Cycle Synchronization with Countercurrent Centrifugal Elutriation
  • Turning Cells into Reagents: The Preparation of Assay Ready Cells for Routine Use in Bioassays
  • Screening of Media Supplements for High-Performance Perfusion Cultures by Design of Experiment
  • ambr® 15 Microbioreactors for CHO Cell Cultivation
  • Using a Parallel Micro-Cultivation System (Micro-Matrix) as a Process Development Tool for Cell Culture Applications
  • HEK293 Cell-Based Bioprocess Development at Bench Scale by Means of On-Line Monitoring in Shake Flasks (RAMOS and SFR)
  • Orbitally Shaken Single-Use Bioreactor for Animal Cell Cultivation: Fed-Batch and Perfusion Mode
  • Development of Mammalian Cell Perfusion Cultures at Lab Scale: From Orbitally Shaken Tubes to Benchtop Bioreactors
  • Perfusion Control for High Cell Density Cultivation and Viral Vaccine Production
  • How to Produce mAbs in a Cube-Shaped Stirred Single-Use Bioreactor at 200 L Scale
  • Generic Workflow for the Setup of Mechanistic Process Models
  • Estimation of Process Model Parameters
  • Efficient Optimization of Process Strategies with Model-Assisted Design of Experiments
  • Design, Optimization, and Adaptive Control of Cell Culture Seed Trains
  • High-Throughput Quantification and Glycosylation Analysis of Antibodies using Bead-Based Assays
  • Surface Plasmon Resonance-Based Method for Rapid Product Sialylation Assessment in Cell Culture
  • Analysis of Product Quality of Complex Polymeric IgM Produced by CHO Cells
  • Raman Trapping Microscopy for Non-Invasive Analysis of Biological Samples
  • An Optical Biosensor for Continuous Glucose Monitoring in Animal Cell Cultures
  • Turbidimetry and Dielectric Spectroscopy as Process Analytical Technologies for Mammalian and Insect Cell Cultures
  • Continuous Chromatography Purification of Virus-Based Biopharmaceuticals: A Shortcut Design Method
  • Single Step Purification of Glycogen Synthase Kinase Isoforms from Small Scale Transient Expression in HEK293 Cells with a Calcium-Dependent Fragment Complementation System.