Cytoskeleton Dynamics [E-Book] : Methods and Protocols / edited by Helder Maiato.
Maiato, Helder, (editor)
1st edition 2020.
New York, NY : Humana Press, 2020
XI, 390 pages 84 illustrations, 72 illustrations in color (online resource)
Methods in Molecular Biology ; 2101
Full Text
Table of Contents:
  • Purification of Mammalian Tubulins and Tubulin-Associated Proteins using a P2A-based Expression System
  • Expression and Purification of Microtubule Associated Proteins from HEK293T Cells for In Vitro Reconstitution
  • In vitro Reconstitution Assays of Microtubule Amplification and Lattice Repair by the Microtubule Severing Enzymes
  • In vitro Microtubule Dynamics Assays using Darkfield Microscopy
  • In vitro Reconstitution of Dynamic Co-organization of Microtubules and Actin Laments in Emulsion Droplets
  • Studying Tau-microtubule Interaction using Single Molecule TIRF Microscopy
  • In Vitro FRET- and Fluorescence-Based Assays to Study Protein Conformation and Protein-Protein Interactions in Mitosis
  • Methods for Studying Membrane-associated Bacterial Cytoskeleton Proteins in vivo by TIRF Microscopy
  • Processing TIRF Microscopy Images to Characterize the Dynamics and Morphology of Bacterial Actin-like Assemblies
  • Studying Centriole Duplication and Elongation in Human Cells
  • Assaying Microtubule Nucleation
  • Detection of Microtubule Nucleation Hotspots at the Golgi
  • Ultrastructural Analysis of Microtubule Ends
  • Optogenetic Control of Microtubule Dynamics
  • Measurement of Microtubule Half-Life and Poleward Flux in the Mitotic Spindle by Photoactivation of Fluorescent Tubulin
  • Functional Dissection of Mitosis using Immortalized Fibroblasts from the Indian Muntjac, A Placental Mammal with only Three Chromosomes
  • Visualization and Functional Analysis of Spindle Actin and Chromosome Segregation in Mammalian Oocytes
  • Using the Four-cell C. elegans Embryo to Study Contractile Ring Dynamics during Cytokinesis
  • Knocking out Multiple Genes in Cultured Primary Neurons to Study Tubulin Posttranslational Modifications
  • Measuring the Role of Tubulin Posttranslational Modifications on Axonal Transport
  • Methods to Measure Perinuclear Actin Dynamics during Nuclear Movement in Migrating Cells. .