MERS Coronavirus [E-Book] : Methods and Protocols / edited by Rahul Vijay.
Vijay, Rahul, (editor)
1st edition 2020.
New York, NY : Humana Press, 2020
XI, 224 pages 40 illustrations, 27 illustrations in color (online resource)
Methods in Molecular Biology ; 2099
Full Text
Table of Contents:
  • Studying Evolutionary Adaptation of MERS-CoV
  • Evaluating MERS-CoV Entry Pathways
  • Biochemical Characterization of Middle East Respiratory Syndrome Coronavirus Spike Protein Proteolytic Processing
  • Crystallization and Structural Determination of the Receptor-binding Domain of MERS-CoV Spike Glycoprotein
  • Bacterial Artificial Chromosome-based Lambda Red Recombination with the I-SceI Homing Endonuclease for Genetic Alteration of MERS-CoV
  • Deducing the Crystal Structure of MERS-CoV Helicase
  • Antigen Capture Enzyme-linked Immunosorbent Assay for Detecting Middle East Respiratory Syndrome Coronavirus in Humans
  • Quantification of the Middle East Respiratory Syndrome-Coronavirus RNA in Tissues by Quantitative Real-Time RT-PCR
  • Evaluation of MERS-CoV Neutralizing Antibodies in Sera Using Live Virus Microneutralization Assay
  • Generation of MERS-CoV Pseudotyped Viral Particles for the Evaluation of Neutralizing Antibodies in Mammalian Sera
  • Qualitative and Quantitative Determination of MERS-CoV S1-specific Antibodies using ELISA
  • Genetically Engineering a Susceptible Mouse Model for MERS-CoV-Induced Acute Respiratory Distress Syndrome
  • Development of a Mouse Adapted MERS Coronavirus
  • Metabolite, Protein, and Lipid Extraction (MPLEx): A Method that Simultaneously Inactivates Middle East Respiratory Syndrome Coronavirus and Allows Analysis of Multiple Host Cell Components Following Infection
  • Evaluation of Activation and Inflammatory Activity of Myeloid Cells DuringPathogenic Human Coronavirus Infection
  • Histopathologic Evaluation and Scoring of Viral Lung Infection. .