RNA interference technology : from basic science to drug development [E-Book] / edited by Krisharao Appasani.
Appasani, Krishnarao, (editor)
Cambridge : Cambridge University Press, 2004
1 online resource (xxxiii, 510 pages)
englisch
9780521205177
9780521836777
9780511546402
Full Text
Table of Contents:
  • Sect. 1. Basic RNAi, siRNA, microRNAs and gene-silencing mechanisms. RNAi beginnings. Overview of the pathway in C. elegans
  • Dicer in RNAi: its roles in vivo and utility in vitro
  • Genes required for RNA interference
  • MicroRNAs: a small contribution from worms
  • miRNAs in the brain and the application of RNAi to neurons
  • Sect. 2. Design, synthesis of siRNAs. Degign and synthesis of small interfering RNA (siRNA)
  • Automated design and high throughput chemical synthesis of siRNA
  • Rational design of siRNAs with the Sfold software
  • Enzymatic production of small interfering RNAs
  • Sect. 3. Vector development and in vivo, in vitro and in ovo delivery methods. Six methods of inducing RNAi in mammalian cells
  • Viral delivery of shRNA
  • siRNA delivery by lentiviral vectors: design and applications
  • Liposomal delivery of siRNAs in mice.
  • Chemical modifications to achieve increased stability and sensitive detection of siRNA
  • RNA interference in postimplantation mouse embryos
  • In ovo RNAi opens new possibilities for functional genomics in vertebrates
  • Sect. 4. Gene silencing in model organisms. Practical applications of RNAi in C. elegans
  • Inducible RNAi as a forward genetic tool in Trypanosoma brucei
  • RNA-mediated gene silencing in fission yeast
  • RNA silencing in filamentous fungi: Mucor ciccinelloides as a model organism
  • RNAi and gene silencing phenomena mediated by viral suppressors in plants.
  • Sect. 5. Drug target validation. Delivering siRNA in vivo for functional genomics and novel therapeutics
  • The role of RNA interference in drug target validation: application to Hepatitis C
  • RNAi in the drug discovery process
  • RNA interference technology in the discovery and validation of druggable targets
  • Sect. 6. Therapeutic and drug development. RNAi-mediated silencing of viral gene expression and replication
  • RNAi in drug development: practical considerations
  • RNA interference studies in liver failure
  • RNAi applications in living animal systems
  • Sect. 7. High-throughput genome-wide RNAi analysis. High-throughput RNAi by soaking in Caenorhabtis elegans
  • Tools for integrative genomics: genome-wide RNAi and expression profiling in Drosophila
  • Microarray analysis and RNA silencing to determine genes functionally important in mesothelioma
  • High-throughput RNA interference
  • Generation of highly specific vector-based shRNAi libraries directed against the entire human genome.