Pollen and Pollen Tube Biology [E-Book] : Methods and Protocols / edited by Anja Geitmann.
Geitmann, Anja, (editor)
1st edition 2020.
New York, NY : Humana Press, 2020
XIII, 332 pages 97 illustrations, 90 illustrations in color (online resource)
Methods in Molecular Biology ; 2160
Full Text
Table of Contents:
  • Pollen Grain Counting Using a Cell Counter
  • A Toolkit for teasing Apart the Early Stages of Pollen-Stigma Interactions in Arabidopsis thaliana
  • Decapitation Crosses to Test Pollen Fertility Mutations for Defects in Stigma-Style Penetration
  • Isolation of the Pistil-Stimulated Pollen Tube Secretome
  • Restricted Pollination for Tracing Individual Pollen Tubes in a Pistil
  • Semi-In Vivo Assay for Pollen Tube Attraction
  • Isolation and Cloning of Suppressor Mutants with Improved Pollen Fertility
  • Workflow to Characterize Mutants with Reproductive Defects
  • Internally-Controlled Methods to Quantify Pollen Tube Growth and Penetration Defects in Arabidopsis thaliana
  • Arabidopsis thaliana Pollen Tube Culture for Multi-Omics Studies
  • Large Scale Analysis of Pollen Viability and Oxidative Level using H2DCFDA-Staining Coupled with Flow Cytometry
  • Obtaining Mutant Pollen for Phenotypic Analysis and Pollen Tube Dual-Staining
  • Galvanotropic Chamber for Controlled Reorientation of Pollen Tube Growth and Simultaneous Confocal Imaging of Intracellular Dynamics
  • Analyzing Intracellular Gradients in Pollen Tubes
  • Silicone Chambers for Pollen Tube Imaging in Microstructures In Vitro Environments
  • Analyzing the Impact of Protein Overexpression on Ca2+ Dynamics and Development in Tobacco Pollen Tubes
  • Imaging and Analysis of the Content of Callose, Pectin, and Cellulose in the Cell Wall of Arabidopsis Pollen Tubes Grown In Vitro
  • Characterization of Growth Behavior and the Resulting Forces Applied by Pollen Tubes in a 3D Matrix
  • Flow Chamber Assay to Image the Response of FRET-Based Nanosensors in Pollen Tubes to Changes in Medium Composition
  • Quantification of Mechanical Forces and Physiological Processes Involved in Pollen Tube Growth Using Microfluidics and Microrobotics
  • Measuring Exocytosis Rate in Arabidopsis Pollen Tube Using Corrected Fluorescence Recovery after Photoconversion (cFRAPc) Technique
  • Testing Pollen Tube Proteins for In Vivo Binding to Phosphatidic Acid by n0Butanol Treatment and Confocal Microscopy.