Dynamin Superfamily GTPases [E-Book] : Methods and Protocols / edited by Rajesh Ramachandran.
Ramachandran, Rajesh, (editor)
1st edition 2020.
New York, NY : Humana Press, 2020
X, 221 pages 53 illustrations, 33 illustrations in color (online resource)
Methods in Molecular Biology ; 2159
Full Text
Table of Contents:
  • Isolation and Analysis of Mitochondrial Fission Enzyme DNM1 from Saccharomyces cerevisiae
  • Purification of the Dynamin-related Protein Vps1 using Mammalian and Bacterial Expression Systems
  • A Single Common Protocol for the Expression and Purification of Soluble Mammalian DSPs from Escherichia coli
  • Affinity Purification and Functional Characterization of Dynamin-related Protein 1
  • Purification and Characterization of MxB
  • Purification of Farnesylated hGBP1 and Characterization of Its Polymerization and Membrane Binding
  • Microscale Thermophoresis (MST) as a Tool for Measuring Dynamin Superfamily Protein (DSP)-lipid Interactions
  • Nucleotide-dependent Dimerization and Conformational Switching of Atlastin
  • Analysis of Mitochondrial Membrane Fusion GTPase OPA1 Expressed by the Silkworm Expression System
  • Cell-free Analysis of Mitochondrial Fusion by Fluorescence Microscopy
  • Electrophysiological Methods For Detection Of Membrane Leakage and Hemifission by Dynamin 1
  • Integrating Optical and Electrochemical Approaches to Assess the Actions of Dynamin at the Fusion Pore
  • Cellular Assays for Measuring Dynamin Activity in Muscle Cells
  • Measuring Drp1 Activity in Mitochondrial Fission in vivo
  • Quantifying Drp1-mediated Mitochondrial Fission by Immunostaining in Fixed Cells
  • Imaging Dynamin-related Protein 1 (Drp1)-mediated Mitochondrial Fission in Living Cells. .