Immunometabolism [E-Book] : Methods and Protocols / edited by Suresh Mishra.
Mishra, Suresh, (editor)
1st edition 2020.
New York, NY : Humana Press, 2020
XVI, 284 pages 58 illustrations, 48 illustrations in color (online resource)
Methods in Molecular Biology ; 2184
Full Text
Table of Contents:
  • Single-Cell Transcriptomics of Immune Cells: Cell Isolation and cDNA Library Generation for scRNA-Seq
  • Monitoring Lactate Dynamics in Individual Macrophages with a Genetically-Encoded Probe
  • The Conjugation of Antibodies for the Simultaneous Detection of Surface Proteins and Transcriptome Analysis at a Single-Cell Level
  • Cellular Fatty Acid Analysis in Macrophage Using Stable Isotope Labeling
  • Proteomic Network Analysis of Polarized Macrophages
  • The Purification and Characterization of Exosomes from Macrophages
  • The Isolation and Characterization of T Lymphocyte Exosomes Using Mass Spectrometry
  • Analysis of Immune-Tumor Cell Interactions Using a 3D Co-Culture Model
  • Studying Adipocyte and Immune Cell Crosstalk Using a Co-Culture System
  • A Gene Expression Analysis of M1 and M2 Polarized Macrophages
  • Simultaneous, Quantitative Characterization of Protein ADP-Ribosylation and Protein Phosphorylation in Macrophages
  • The Analysis of Mycobacterium tuberculosis-Induced Bioenergetic Changes in Infected Macrophages Using an Extracellular Flux Analyzer
  • Analyzing the Metabolic Phenotype of Bone Marrow-Derived Dendritic Cells by Assessing Their Oxygen Consumption and Extracellular Acidification
  • The Evaluation of Mitochondrial Membrane Potential Using Fluorescent Dyes or a Membrane-Permeable Cation (TPP+) Electrode in Isolated Mitochondria and Intact Cells
  • The Multiparametric Analysis of Mitochondrial Dynamics in T Cells from Cryopreserved Peripheral Blood Mononuclear Cells (PBMCs)
  • The Measurement of Whole Body Glucose Homeostasis in Mice
  • Immunometabolism and Its Potential to Improve the Current Limitations of Immunotherapy
  • Sex Differences in Immunometabolism: An Unexplored Area
  • Isolation and Preparation of Bone Marrow-Derived Immune Cells for Metabolic Analysis.