DNA Cloning and Assembly [E-Book] : Methods and Protocols / edited by Sunil Chandran, Kevin W. George.
Chandran, Sunil, (editor)
George, Kevin W., (editor)
1st edition 2020.
New York, NY : Humana Press, 2020
XIII, 334 pages 81 illustrations, 69 illustrations in color (online resource)
Methods in Molecular Biology ; 2205
Full Text
Table of Contents:
  • An Integrated Computer-Aided Design and Manufacturing Workflow for Synthetic Biology
  • Combinatorial-Hierarchical DNA Library Design Using the TeselaGen DESIGN Module with j5
  • The AssemblX Toolkit for Reliable and User-friendly Multi-gene Assemblies
  • High-Throughput PCR for DNA Part Generation
  • High-Throughput DNA Assembly Using Yeast Homologous Recombination
  • Highly-Multiplexed, Semi-Automated Nextera Next Generation Sequencing (NGS) Library Preparation
  • Generation of MoClo Standard Parts Using Golden Gate Cloning
  • Assembly of Multigene Constructs using the Modular Cloning System MoClo
  • Hierarchical Modular DNA Assembly using MetClo
  • PaperClip DNA Assembly: Reduce, Reuse, Recycle
  • Phytobricks: Manual and Automated Assembly of Constructs for Engineering Plants
  • Mobius Assembly
  • Design and Implementation of Multi-Protein Expression Constructs and Combinatorial Libraries using Start-Stop Assembly
  • BASIC - A Simple and Accurate Modular DNA Assembly Method
  • Joint Universal Modular Plasmids: A Flexible Platform for Golden Gate Assembly in Any Microbial Host
  • A Step-by-Step Protocol for COMPASS, a Synthetic Biology Tool for Combinatorial Gene Assembly
  • SCRaMbLE-in: A Fast and Efficient Method to Diversify and Improve the Yields of Heterologous Pathways in Synthetic Yeast. .