Homologous Recombination [E-Book] : Methods and Protocols / edited by Andrés Aguilera, Aura Carreira.
Aguilera, Andrés, (editor)
Carreira, Aura, (editor)
1st edition 2021.
New York, NY : Humana Press, 2021
XV, 557 pages 126 illustrations, 91 illustrations in color (online resource)
Methods in Molecular Biology ; 2153
Full Text
Table of Contents:
  • Detection of DNA Double Strand Breaks by -H2AX Immunodetection
  • END-seq: An Unbiased, High-Resolution and Genome-Wide Approach to Map DNA Double Strand Breaks and Resection in Human Cells
  • Resection of a DNA Double-Strand Break by Alkaline Gel Electrophoresis and Southern Blotting
  • Analysis of DNA Double-Strand Break Ends Resection and Single-Strand Annealing in S. pombe
  • Quantifying DNA End Resection in Human Cells
  • Genetic and Molecular approaches to Study Chromosomal Breakage at Secondary Structure-Forming Repeats
  • Biochemical analysis of D-Loop Extension and DNA Strand Displacement Synthesis
  • DNA Strand Exchange to Monitor Human RAD51-Mediated Strand Invasion and Pairing
  • Monitoring Homologous Recombination Activity in Human Cells
  • Inter-Homolog Homologous Recombination in Mouse Embryonic Stem Cells
  • Branch Migration Activity of Rad54 Protein
  • Holliday Junction Resolution
  • Identification and Analysis of Different Types of UFBs
  • Intrachromosomal Recombination in Yeast
  • Genome-Wide Analysis of Mitotic Recombination in Budding Yeast
  • Monitoring Gene Conversion in Budding Yeast by Southern Blot Analysis
  • DNA Double-Strand Break Induced Gene Amplification in Yeast
  • Measuring Chromosome Pairing during Homologous Recombination in Yeast
  • Cytological Monitoring of Meiotic Crossovers in Spermatocytes and Oocytes
  • Detection of DSBs in C. elegans Meiosis
  • Methods to Map Meiotic Recombination Proteins in Saccharomyces cerevisiae
  • Investigation of Break-Induced Replication in Yeast
  • Measurement of Homologous Recombination at Stalled Mammalian Replication Forks
  • Super Resolution Imaging of Homologous Recombination Repair at Collapsed Replication Forks
  • The Analysis of Recombination-Dependent Processing of Blocked Replication Forks by Bi-Dimensional Gel Electrophoresis
  • The Sister Chromatid Exchange Assay in Human Cells
  • Analysis of Recombination at Yeast Telomeres
  • Gel Electrophoresis Analysis of rDNA Instability in Saccharomyces cerevisiae
  • Analyzing Homologous Recombination at a Genome Wide Level
  • CRISPR/Cas9 Induced Breaks in Heterochromatin, Visualized by Immunofluorescence
  • In Vivo Binding of Recombination Proteins of Non-DSB DNA Lesions to Replication Forks
  • Live Cell Imaging of Nuclear Actin Filaments and Heterochromatic Repair foci in Drosophila and Mouse Cells
  • In Vitro Characterization of Sumoylation of HR Proteins
  • High-Throughput Analysis of Heteroduplex DNA in Mitotic Recombination Products
  • Fluorescence Microscopy for Analysis of Relocalization of Structure-Specific Endonucleases
  • Physical and Genetic Assays for the Study of DNA Joint Molecules Metabolism and Multi-Invasion-Induced Rearrangements in S. cerevisiae.