CRISPR Guide RNA Design [E-Book] : Methods and Protocols / edited by Tudor A. Fulga, David J. H. F. Knapp, Quentin R. V. Ferry.
This detailed volume focuses on the CRISPR-associated guide RNA and how it can be designed, modified, and validated for a broad repertoire of purposes. Beginning with a section on computational design of target-specific guide RNAs, the book continues by covering chemical modifications to alter guide...
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Full text |
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Personal Name(s): | Ferry, Quentin R. V., editor |
Fulga, Tudor A., editor / Knapp, David J. H. F., editor | |
Edition: |
1st edition 2021. |
Imprint: |
New York, NY :
Humana Press,
2021
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Physical Description: |
X, 286 pages 56 illustrations, 46 illustrations in color (online resource) |
Note: |
englisch |
ISBN: |
9781071606872 |
DOI: |
10.1007/978-1-0716-0687-2 |
Series Title: |
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Methods in Molecular Biology ;
2162 |
Subject (ZB): | |
Subject (LOC): | |
Classification: |
- Cloud-Based Design of Short Guide RNA (sgRNA) Libraries for CRISPR Experiments
- Web-Based CRISPR Toolkits: Cas-OFFinder, Cas-Designer, and Cas-Analyzer
- Chemical Modification of Guide RNAs for Improved CRISPR Activity in CD34+ Human Hematopoietic Stem and Progenitor Cells
- Gene Disruption Using Chemically-Modified CRISPR-Cpf1 RNA
- 'Split-and-Click' sgRNA
- Chimeric DNA-RNA Guide RNA Designs
- Harnessing tRNA for Processing Ability and Promoter Activity
- Targeting Noncoding RNA Domains to Genomic Loci with CRISPR-Display: Guidelines for Designing, Building, and Testing sgRNA-ncRNA Expression Constructs
- Controlling the Activity of CRISPR Transcriptional Regulators with Inducible sgRNAs
- Gene Manipulation Using Fusion Guide RNAs for Cas9 and Cas12a
- Methods for Measuring CRISPR/Cas9 DNA Cleavage in Cells
- In Vitro Assays for Comparing the Specificity of First- and Next-Generation CRISPR/Cas9 Systems
- Profiling Genome-Wide Specificity of CRISPR-Cas9 Using Digenome-Seq
- Detection of CRISPR/Cas9-Generated Off-Target Effect by Integration-Defective Lentiviral Vector
- Genome-Wide CRISPR Off-Target DNA Break Detection by the BLISS Method.