Glycosaminoglycans [E-Book] : Methods and Protocols / edited by Kuberan Balagurunathan, Hiroshi Nakato, Umesh Desai, Yukio Saijoh.
This second edition volume expands on the previous edition with new information on the structural analysis of glycosaminoglycans (GAGs); chemical and enzymatic synthesis of GAGs; biophysical, biochemical, and computational analysis of GAG-protein interactions; molecular and genetic approaches to man...
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Full text |
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Personal Name(s): | Balagurunathan, Kuberan, editor |
Desai, Umesh, editor / Nakato, Hiroshi, editor / Saijoh, Yukio, editor | |
Edition: |
2nd edition 2022. |
Imprint: |
New York, NY :
Humana Press,
2022
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Physical Description: |
XXI, 829 pages 180 illustrations, 114 illustrations in color (online resource) |
Note: |
englisch |
ISBN: |
9781071613986 |
DOI: |
10.1007/978-1-0716-1398-6 |
Series Title: |
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Methods in Molecular Biology ;
2303 |
Subject (LOC): |
- One-Pot Enzymatic Synthesis of Heparin from N-Sulfoheparosan
- Solution NMR Spectroscopy for Characterizing Protein-Glycosaminoglycan Interactions
- Metabolic Labeling of Proteoglycans and Analysis of Their Synthesis and Sorting in Filter-Grown and Polarized Epithelial Cells
- Fluorous-Tag Assisted Synthesis of GAG-Like Oligosaccharides
- Aqueous Molecular Dynamics for Understanding Glycosaminoglycan Recognition by Proteins
- A Non-Radioactive Method to Measure Hyaluronan Synthase Activity
- A Glycoproteomic Approach to Identify Novel Proteoglycans
- Analysis of Protein-Glycosaminoglycan Interactions using Traveling Wave Ion Mobility Mass Spectrometry
- Ascidian (Chordata-Tunicata) Glycosaminoglycans: Extraction, Purification, Biochemical, and Spectroscopic Analysis
- Quantitative Disaccharide Profiling of Glycosaminoglycans from Two Different Preparations by PMP and Deuterated PMP Labeling
- Preparation and Characterization of Heparan Sulfate Derived Oligosaccharides to Investigate Protein-GAG Interaction and HS Biosynthesis Enzyme Activity
- Heparan Sulfate Structure: Methods to Study N-sulfation and NDST Action
- Methods for the Production of Recombinant Heparosan: A Critical Heparin Precursor, from Non-Pathogenic E.coli Strains
- De novo Sequencing of Heparin/Heparan Sulfate Oligosaccharides by Chemical Derivatization and LC-MS/MS
- Production and HPLC-Based Disaccharide Analysis of Xyloside-Primed Glycosaminoglycans
- Nuclear Magnetic Resonance Methods in Structural Characterization of Glycosaminoglycans
- Aggrecan: Approaches to Study Biophysical and Biomechanical Properties
- Chromatographic Molecular Weight Measurements for Heparin: Its Fragments and Fractions, and Other Glycosaminoglycans
- Mass Spectrometric Methods for the Analysis of Heparin and Heparan Sulfate
- Affinity Purification of Glycosylphosphatidylinositol-Anchored Proteins by Alpha-Toxin
- Thermodynamic Affinity and Nature of Forces Defining Glycosaminoglycan: Protein Systems Using Fluorescence Spectroscopy
- Isolation and Characterization of Heparan Sulfate Containing Amyloid Precursor Protein Degradation Products
- Chemical Approaches to Prepare Modified Heparin and Heparosan Polymers for Biological Studies
- Glycosaminoglycan Quality Control by Monosaccharide Analysis
- Characterizing Thermodynamics of Protein-Glycosaminoglycan Interactions using Isothermal Titration Calorimetry
- Automated Synthesis of Chondroitin Sulfate Oligosaccharides
- Capillary Electrophoretic Analysis of Isolated Sulfated Polysaccharides to Characterize Pharmaceutical Products
- Preparation of Isotope Enriched Heparan Sulfate Precursors for Structural Biology Studies
- Methods for Measuring Exchangeable Protons in Glycosaminoglycans
- Advances in Studying Glycosaminoglycan-Protein Interactions Using Capillary Electrophoresis
- anexVis: A Transcriptome Tool to Visualize Organ/Tissue-Specific Glycosaminoglycan Biosynthetic and Catabolic Pathways in Human Health and Diseases
- Molecular Genetic Techniques for the Proteoglycan Functions in Drosophila
- Measuring Sulfatase Expression and Invasion in Glioblastoma
- Analyzing the Role of Heparan Sulfate Proteoglycans in Axon Guidance In Vivo in Zebrafish
- Regulation of 3-O-Sulfation of Heparan Sulfate during Transition from the Naïve to the Primed State in Mouse Embryonic Stem Cells
- Analysis of Human Hyaluronan Synthase Gene Transcriptional Regulation and Downstream Hyaluronan Cell Surface Receptor Mobility in Myofibroblast Differentiation
- Methods to Analyze the Effect of Diet-Derived Metabolites on Endothelial Inflammation and Cell Surface Glycosaminoglycans
- Assays for Evaluation of Substrates for and Inhibitors of β-1,4-Galactosyltransferase 7
- Enzymatic Alteration of ECM to Explore Muscle Function and Motor Control of a Learned Behavior
- Procedures to Evaluate the Role of Heparan Sulfate in the Reactivity of Resistance and Conductance Arteries Ex Vivo
- Computerized Molecular Modeling for Discovering Promising Glycosaminoglycan Oligosaccharides that Modulate Protein Function
- Imaging Glycosaminoglycan Modification Patterns In Vivo
- Isolation and Purification of Versican and Analysis of Versican Proteolysis
- Identification of Cell Autonomous and Non-Cell Autonomous Functions of Heparan Sulfate Glycosaminoglycan Chains by Creating Chimeric Mouse Embryos
- Methods for Assessing the Effects of Xylosides on Angiogenesis
- Role of HSPGs in Systemic Bacterial Infections
- Generation of Drosophila Heparan Sulfate Mutant Cell Lines from Existing Fly Strains
- Application of a Mutant Cell Library to Determine the Structure-Function Relationship of Heparan Sulfate in Facilitating FGF-FGFR Signaling
- Manipulation of Glycosaminoglycans using Synthetic Xylosides to Study their Roles in Lung Branching Morphogenesis in Ex Vivo Lung Bud Culture System
- Informatics Ecosystems to Advance the Biology of Glycans
- Analysis of 3'-phosphoadenosine 5'-phosphosulfate Transporters: Transporter Activity Assay, Real-Time Reverse Transcription Polymerase Chain Reaction, and Immunohistochemistry
- Preparation of Nanosensors for Detecting the Activity of Glycosaminoglycan Cleaving Enzymes
- The Detection of Glycosaminoglycans in Pancreatic Islets and Lymphoid Tissues
- Histochemical Analysis of Heparan Sulfate 3-O-sulfotransferase Expression in Mouse Brain
- Mapping Proteoglycan Functions Using Novel Genetic Strategies
- Xyloside Derivatives as Molecular Tools to Selectively Inhibit Heparan Sulfate and Chondroitin Sulfate Proteoglycan Biosynthesis
- A Strategic Approach to Identification of Selective Inhibitors of Cancer Stem Cells
- Applications of Xylosides in the Manipulation of Stem Cell Niche to Regulate Human Neural Stem Cell Differentiation and Neurite Outgrowth
- Murine Models in the Evaluation of Heparan Sulfate-Based Anticoagulants
- Investigating the Roles of Heparan Sulfate Structures in Alpha-Synuclein Aggregation in Cell Culture Models.