Transposable Elements [E-Book] : Methods and Protocols / edited by Miguel R. Branco, Alexandre de Mendoza Soler.
The volume presents a small selection of state-of-the-art approaches for studying transposable elements(TE). Chapters guide readers through HTS-based approaches, bioinformatic tools, methods to studyTE protein complexes, and the functional impact on the host. Written in the successful Methods in Mol...
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Personal Name(s): | Branco, Miguel R., editor |
de Mendoza Soler, Alexandre, editor | |
Edition: |
1st edition 2023. |
Imprint: |
New York, NY :
Humana Press,
2023
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Physical Description: |
XII, 382 pages 65 illustrations, 60 illustrations in color (online resource) |
Note: |
englisch |
ISBN: |
9781071628836 |
DOI: |
10.1007/978-1-0716-2883-6 |
Series Title: |
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Methods in Molecular Biology ;
2607 |
Subject (LOC): |
- An overview of best practices for transposable element identification, classification and annotation in eukaryotic genomes
- Assembly-free annotation and quantification of transposable elements with dnaPipeTE
- Best practice for the identification of horizontally transferred transposons
- Genotyping of transposable elements insertions segregating in the human populations using short-read re-alignments
- A Pangenome approach to detect and genotype TE insertion polymorphisms.0 Experimental validation of transposable element insertions using the Polymerase Chain Reaction (PCR)
- Quantification of LINE-1 RNA expression from bulk RNA-seq using L1EM
- Genome-wide profiling of L1 DNA methylation by bs-ATLAS-seq
- Nanopore epigenomic analysis of transposable element DNA modifications
- Targeted resequencing and methylation analysis of L1 elements by nanopore sequencing
- Inferring protein-DNA binding profiles at interspersed repeats using HiChIP and PatChER
- Affinity-based Interactome Analysis of Endogenous LINE-1 Macromolecules
- LINE-1 retrotransposition assays in embryonic stem cells
- Detecting somatic transposable element insertions in Drosophila tissues
- Precise and scarless insertion of transposable elements by Cas9-mediated genome engineering
- Epigenetic editing of transposable and repetitive elements
- Using CRISPR to investigate the regulatory activity of transposable elements. .