Skeletal Muscle Stem Cells [E-Book] : Methods and Protocols /

Skeletal Muscle Stem Cells [E-Book] : Methods and Protocols / edited by Atsushi Asakura

This volume looks at the latest technologies and methods--combined with new genetic tools available in animal models--used in this constantly evolving field. The chapters in this book are organized into three sections: Section one covers muscle stem cells and progenitor cells; Section Two discusses...

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Asakura, Atsushi, editor
1st edition 2023.
New York, NY : Humana Press, 2023
XV, 481 pages 126 illustrations, 116 illustrations in color (online resource)
englisch
9781071630365
10.1007/978-1-0716-3036-5
Methods in Molecular Biology ; 2640
Stem cells.



  • Flow Cytometer Analyses, Isolation, and Staining of Murine Muscle Satellite Cells
  • Extra Eyelid-Derived Muscle Stem Cells
  • Isolation, Culture, and Analysis of Zebrafish Myofibres and Associated Muscle Stem Cells to Explore Adult Skeletal Myogenesis
  • The Satellite Cell Colony Forming Cell Assay as a Tool to Measure Self-Renewal and Differentiation Potential
  • Co-Cultures of Macrophages with Muscle Stem Cells or with Fibroadipogenic Precursor Cells from Regenerating Skeletal Muscle
  • Measuring Oxygen Consumption Rate (OCR) and Extracellular Acidification Rate (ECAR) in Muscle Stem Cells Using a Seahorse Analyzer: Applicability for Aging Studies
  • High Throughput Screening of Mitochondrial Bioenergetics in Myoblasts and Differentiated Myotubes
  • State of the Art Procedures for the Isolation and Characterization of Mesoangioblasts
  • Analyses of Mesenchymal Progenitors in Skeletal Muscle by Fluorescence-Activated Cell Sorting and Tissue Clearing
  • In Vitro Maturation of Human Pluripotent Stem Cell-Derived Myotubes
  • Differentiation of Human Fetal Muscle Stem Cells from Induced Pluripotent Stem Cells
  • Sphere-Based Expansion of Myogenic Progenitors from Human Pluripotent Stem Cells
  • Producing Engraftable Skeletal Myogenic Progenitors from Pluripotent Stem Cells via Teratoma Formation
  • Techniques for Injury, Cell Transplantation, and Histological Analysis in Skeletal Muscle
  • Murine Models of Tenotomy-Induced Mechanical Overloading and Tail-Suspension-Induced Mechanical Unloading
  • Skeletal Muscle Denervation: Sciatic and Tibial Nerve Transection Technique
  • Skeletal Muscle Regeneration in Zebrafish
  • Methods to Monitor Circadian Clock Function in Skeletal Muscle
  • Visualizing MyoD Oscillations in Muscle Stem Cells
  • In Vivo Modeling of Skeletal Muscle Diseases Using the CRISPR/Cas9 System in Rats
  • In Vivo Investigation of Gene Function in Muscle Stem Cells by CRISPR/Cas9 Mediated Genome Editing
  • Exons 45-55 Skipping Using Antisense Oligonucleotides in Immortalized Human DMD Muscle Cells
  • In Vivo Evaluation of Exon 51 Skipping in hDMD/Dmd-null Mice
  • Functional Analysis of MicroRNAs in Skeletal Muscle
  • Targeted Lipidomics Analysis of Adipose and Skeletal Muscle Tissues by Multiple Reaction Monitoring (MRM) Profiling
  • Single-Cell Transcriptomic Analysis of Mononuclear Cell Populations in Skeletal Muscle
  • Assay for Transposase-Accessible Chromatin Using Sequencing (ATAC-seq) of Freshly Isolated Muscle Stem Cells
  • Efficient Genome-Wide Chromatin Profiling by CUT&RUN with Low Numbers of Muscle Stem Cells
  • Epitranscriptome Mapping of N6-Methyladenosine using m6A Immunoprecipitation with High Throughput Sequencing in Skeletal Muscle Stem Cells
  • Visualization of RNA Transcripts in Muscle Stem Cells Using Single-Molecule Fluorescence In Situ Hybridization
  • Tissue Clearing and Confocal Microscopic Imaging for Skeletal Muscle
  • Three-Dimensional Imaging Analysis for Skeletal Muscle.