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This title appears in the Scientific Report : 2010 

Novel Fusogenic Liposomes for Fluorescent Cell labeling and Membrane Modification

Novel Fusogenic Liposomes for Fluorescent Cell labeling and Membrane Modification

Efficient delivery of biomolecules into membranes of living cells as well as cell surface modifications are major biotechnological challenges. Here, novel liposome systems based on neutral and cationic lipids in combination with lipids modified by aromatic groups are introduced for such applications...

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Personal Name(s): Csiszar, A.
Hersch, N. / Dieluweit, S. / Biehl, R. / Merkel, R. / Hoffmann, B.
Contributing Institute: Biomechanik; IBN-4
Neutronenstreuung; IFF-5
Published in: Bioconjugate chemistry, 21 (2010) S. 537 - 543
Imprint: Columbus, Ohio American Chemical Society 2010
Physical Description: 537 - 543
DOI: 10.1021/bc900470y
PubMed ID: 20184308
Document Type: Journal Article
Research Program: Erneuerbare Energien
BioSoft: Makromolekulare Systeme und biologische Informationsverarbeitung
Series Title: Bioconjugate Chemistry 21
Subject (ZB):
J
Publikationsportal JuSER
Please use the identifier: http://dx.doi.org/10.1021/bc900470y in citations.

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Efficient delivery of biomolecules into membranes of living cells as well as cell surface modifications are major biotechnological challenges. Here, novel liposome systems based on neutral and cationic lipids in combination with lipids modified by aromatic groups are introduced for such applications. The fusion efficiency of these liposome systems was tested on single cells in culture like HEK293, myofibroblasts, cortical neurons, human macrophages, smooth muscle cells, and even on tissue. Fusogenic liposomes enabled highly efficient incorporation of molecules into mammalian cell membranes within 1 to 30 min at fully unchanged cell growth conditions and did not affect cell behavior. We hypothesize that membrane fusions were induced in all cases by the interaction of the positively charged lipids and the delocalized electron system of the aromatic group generating local dipoles and membrane instabilities. Selected applications ranging from basic research to biotechnology are envisaged here.

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