This title appears in the Scientific Report :
2010
Please use the identifier:
http://dx.doi.org/10.1021/bc900470y in citations.
Novel Fusogenic Liposomes for Fluorescent Cell labeling and Membrane Modification
Novel Fusogenic Liposomes for Fluorescent Cell labeling and Membrane Modification
Efficient delivery of biomolecules into membranes of living cells as well as cell surface modifications are major biotechnological challenges. Here, novel liposome systems based on neutral and cationic lipids in combination with lipids modified by aromatic groups are introduced for such applications...
Saved in:
Personal Name(s): | Csiszar, A. |
---|---|
Hersch, N. / Dieluweit, S. / Biehl, R. / Merkel, R. / Hoffmann, B. | |
Contributing Institute: |
Biomechanik; IBN-4 Neutronenstreuung; IFF-5 |
Published in: | Bioconjugate chemistry, 21 (2010) S. 537 - 543 |
Imprint: |
Columbus, Ohio
American Chemical Society
2010
|
Physical Description: |
537 - 543 |
DOI: |
10.1021/bc900470y |
PubMed ID: |
20184308 |
Document Type: |
Journal Article |
Research Program: |
Erneuerbare Energien BioSoft: Makromolekulare Systeme und biologische Informationsverarbeitung |
Series Title: |
Bioconjugate Chemistry
21 |
Subject (ZB): | |
Publikationsportal JuSER |
Efficient delivery of biomolecules into membranes of living cells as well as cell surface modifications are major biotechnological challenges. Here, novel liposome systems based on neutral and cationic lipids in combination with lipids modified by aromatic groups are introduced for such applications. The fusion efficiency of these liposome systems was tested on single cells in culture like HEK293, myofibroblasts, cortical neurons, human macrophages, smooth muscle cells, and even on tissue. Fusogenic liposomes enabled highly efficient incorporation of molecules into mammalian cell membranes within 1 to 30 min at fully unchanged cell growth conditions and did not affect cell behavior. We hypothesize that membrane fusions were induced in all cases by the interaction of the positively charged lipids and the delocalized electron system of the aromatic group generating local dipoles and membrane instabilities. Selected applications ranging from basic research to biotechnology are envisaged here. |