This title appears in the Scientific Report :
2012
Please use the identifier:
http://dx.doi.org/10.1021/ja211802z in citations.
Branchpoint expansion in a fully-complementary three-way DNA junction.
Branchpoint expansion in a fully-complementary three-way DNA junction.
Branched nucleic acid molecules serve as key intermediates in DNA replication, recombination, and repair; architectural elements in RNA; and building blocks and functional components for nanoscience applications. Using a combination of high-resolution single-molecule FRET, time-resolved spectroscopy...
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Personal Name(s): | Sabir, T. |
---|---|
Toulmin, A. / Ma, L. / Jones, A.C. / McGlynn, P. / Schröder, G.F. / Magennis, S.W. | |
Contributing Institute: |
Strukturbiochemie; ICS-6 |
Published in: | Journal of the American Chemical Society, 134 (2012) S. 6280 - 6285 |
Imprint: |
Washington, DC
American Chemical Society
2012
|
Physical Description: |
6280 - 6285 |
DOI: |
10.1021/ja211802z |
PubMed ID: |
22329743 |
Document Type: |
Journal Article |
Research Program: |
BioSoft: Makromolekulare Systeme und biologische Informationsverarbeitung Funktion und Dysfunktion des Nervensystems |
Series Title: |
Journal of the American Chemical Society
134 |
Subject (ZB): | |
Publikationsportal JuSER |
Branched nucleic acid molecules serve as key intermediates in DNA replication, recombination, and repair; architectural elements in RNA; and building blocks and functional components for nanoscience applications. Using a combination of high-resolution single-molecule FRET, time-resolved spectroscopy, and molecular modeling, we have probed the local and global structure of a DNA three-way junction (3WJ) in solution. We found that it adopts a Y-shaped, pyramidal structure, in which the bases adjacent to the branchpoint are unpaired, despite the full Watson-Crick complementarity of the molecule. The unpairing allows a nanoscale cavity to form at the junction center. Our structure accounts for earlier observations made of the structure, flexibility, and reactivity of 3WJs. We anticipate that these results will guide the development of new DNA-based supramolecular receptors and nanosystems. |