Description: Inferring spatial organization of bonds within adhesion clusters by exploiting fluctuations of soft interfaces

This title appears in the Scientific Report : 2010

Inferring spatial organization of bonds within adhesion clusters by exploiting fluctuations of soft interfaces

Detecting the organization of bonds within adhesion domains connecting two interacting membranes is, at present, extremely challenging. Herein we present a technique, based on Reflection Interference Contrast Microscopy, which uses spontaneous thermal fluctuations of a soft interface as a tool to id...

Personal Name(s):	Smith, A.-S.
	Fenz, S.F. / Sengupta, K.
Contributing Institute:	Biomechanik; IBN-4
Published in:	epl, 89 (2010) S. 28003
Imprint:	Les Ulis EDP Sciences 2010
Physical Description:	28003
DOI:	10.1209/0295-5075/89/28003
Document Type:	Journal Article
Research Program:	BioSoft: Makromolekulare Systeme und biologische Informationsverarbeitung
Series Title:	EPL : a Letters Journal Exploring the Frontiers of Physics 89
Subject (ZB):	J
	Publikationsportal JuSER

Please use the identifier: http://dx.doi.org/10.1209/0295-5075/89/28003 in citations.

Detecting the organization of bonds within adhesion domains connecting two interacting membranes is, at present, extremely challenging. Herein we present a technique, based on Reflection Interference Contrast Microscopy, which uses spontaneous thermal fluctuations of a soft interface as a tool to identify the organization of specific ligand-receptor bonds. The key is a time-resolved analysis of micro-interferometric data that systematically quantifies fluctuations and enables the detection of their suppression due to the formation of bonds, which, in turn, allows the identification of the bond organization without the use of fluorescent labelling. The identification of a new type of bond organization characterized by sparsely distributed bonds, as well as detection of pinning centres of nanometric size is presented. Copyright (C) EPLA, 2010