This title appears in the Scientific Report :
2014
Please use the identifier:
http://hdl.handle.net/2128/5697 in citations.
Metabolic engineering of $\textit{Corynebacterium glutamicum}$ for production of the adipate precursor 2-oxoadipate
Metabolic engineering of $\textit{Corynebacterium glutamicum}$ for production of the adipate precursor 2-oxoadipate
The C6-dicarboxylic acid adipate is one of the most important building blocks in the chemical industry. The chemical syntheses of adipate are connected to insanitary and environmentally harmful reagents and catalysts. Therefore, the establishment of a biotechnological process for adipate production...
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Personal Name(s): | Spelberg, Markus Sebastian (Corresponding author) |
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Contributing Institute: |
Biotechnologie; IBG-1 |
Published in: | 2014 |
Imprint: |
Jülich
Forschungszentrum Jülich GmbH Zentralbibliothek, Verlag
2014
|
Physical Description: |
118 S. |
Dissertation Note: |
Heinrich-Heine-Universität Düsseldorf, Diss., 2013 |
ISBN: |
978-3-89336-954-6 |
Document Type: |
Dissertation / PhD Thesis |
Research Program: |
ohne Topic |
Series Title: |
Schriften des Forschungszentrums Jülich. Reihe Gesundheit / Health
70 |
Subject (ZB): | |
Link: |
OpenAccess |
Publikationsportal JuSER |
The C6-dicarboxylic acid adipate is one of the most important building blocks in the chemical industry. The chemical syntheses of adipate are connected to insanitary and environmentally harmful reagents and catalysts. Therefore, the establishment of a biotechnological process for adipate production based on renewable carbon sources is of substantial interest. Thus, the main objective of this work was the metabolic engineering of $\textit{Corynebacterium glutamicum}$ for the production of the adipate precursor 2-oxoadipate. This organism is an industrially established producer of amino acids, but was also shown to possess a high capability for the production of organic acids, such as lactate or succinate. Neither adipate nor 2-oxoadipate (solutions adjusted to pH 7) were metabolised by $\textit{C. glutamicum}$ wild type and they did not inhibit growth in glucose minimal medium in concentrations up to 50 mM (μ = 0.43 h$^{-1}$). Addition of 150 mM or 250 mM adipate resulted in a 37% decreased growth rate (μ = 0.29 h$^{-1}$), whereas a concentration of 500 mM adipate almost completely abolished growth. When adjusting the pH of the adipate solution with KOH to pH 7, the addition of 500 mM adipate still allowed a growth rate $\mu$ of 0.09 h$^{-1}$. Additional supplementation of 5 mM of the compatible solute L-proline further increased the growth rate to 0.16 h$^{-1}$. The latter result and DNA microarray experiments performed in the presence of 50 mM and 150 mM adipate indicated that the growth retardation might be causedto some extent by osmotic stress. Furthermore, the transcriptome data revealed high upregulation of the transcriptional regulator PcaR and its target genes $\textit{pcaIJ}$ and $\textit{pcaFDO}$, belonging to the PCA (protocatechuate) branch of the $\beta$-ketoadipate pathway. Thus, adipate probably acts as an activator molecule of PcaR in $\textit{C. glutamicum}$. [...] |