This title appears in the Scientific Report :
2013
On-chip optical stimulation and electrical recording from cells
On-chip optical stimulation and electrical recording from cells
We demonstrate an optoelectrical device capable of bidirectional optical stimulation and electrophysiological recording. It consists of an array of micropixellated InGaN light-emitting diodes coupled to a custom-made ultrathin planar microelectrode array. Electrogenic cells (HL-1) were transfected w...
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Personal Name(s): | Yakushenko, Alexey |
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Maybeck, Vanessa / Boris, Hofmann / Gong, Zheng / Gu, Erdan / Dawson, Martin / Offenhäusser, Andreas / Wolfrum, Bernhard | |
Contributing Institute: |
JARA-FIT; JARA-FIT Bioelektronik; PGI-8 Bioelektronik; ICS-8 |
Imprint: |
2012
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Conference: | 1st International Biophotonics Meeting in Israel, Tel Aviv (Israel), 2012-12-09 - 2012-12-11 |
Document Type: |
Poster |
Research Program: |
Sensorics and bioinspired systems Physics of the Cell Helmholtz Young Investigators Group |
Publikationsportal JuSER |
We demonstrate an optoelectrical device capable of bidirectional optical stimulation and electrophysiological recording. It consists of an array of micropixellated InGaN light-emitting diodes coupled to a custom-made ultrathin planar microelectrode array. Electrogenic cells (HL-1) were transfected with a light-sensitive protein and cultured directly on the chip. We monitored action potentials of individual spontaneously beating HL-1 cells by extracellular electrical recordings. On-chip light stimulation of non-beating HL-1 cells triggered network activity that was recorded using calcium imaging. We see the potential of our chip for electrophysiological experiments of optogenetically-modified cells with single cell resolution without complex optics or external light sources. |