This title appears in the Scientific Report :
2014
Please use the identifier:
http://dx.doi.org/10.1039/c4ib00027g in citations.
Please use the identifier: http://hdl.handle.net/2128/7981 in citations.
Light-responsive control of bacterial gene Expression: precise triggering of the lac promoter activity using photocaged IPTG
Light-responsive control of bacterial gene Expression: precise triggering of the lac promoter activity using photocaged IPTG
Light can be used to control numerous cellular processes including protein function and interaction as well as gene expression in a non-invasive fashion and with unprecedented spatiotemporal resolution. However, for chemical phototriggers tight, gradual, and homogeneous light response has never been...
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Personal Name(s): | Binder, Dennis |
---|---|
Grünberger, Alexander / Loeschcke, Anita / Probst, Christopher / Bier, Claus / Pietruszka, Jörg / Wiechert, Wolfgang / Kohlheyer, Dietrich / Jaeger, Karl-Erich (Corresponding Author) / Drepper, Thomas | |
Contributing Institute: |
Biotechnologie; IBG-1 Institut für Bioorganische Chemie (HHUD); IBOC |
Published in: | Integrative biology, 6 (2014) 8, S. 755-765 |
Imprint: |
Cambridge
RSC Publ.
2014
|
DOI: |
10.1039/c4ib00027g |
PubMed ID: |
24894989 |
Document Type: |
Journal Article |
Research Program: |
ohne Topic |
Link: |
OpenAccess |
Publikationsportal JuSER |
Please use the identifier: http://hdl.handle.net/2128/7981 in citations.
Light can be used to control numerous cellular processes including protein function and interaction as well as gene expression in a non-invasive fashion and with unprecedented spatiotemporal resolution. However, for chemical phototriggers tight, gradual, and homogeneous light response has never been attained in living cells. Here, we report on a light-responsive bacterial T7 RNA polymerase expression system based on a photocaged derivative of the inducer molecule isopropyl-β-D-thiogalactopyranoside (IPTG). We have comparatively analyzed different Escherichia coli lac promoter-regulated expression systems in batch and microfluidic single-cell cultivation. The lacY-deficient E. coli strain Tuner(DE3) harboring additional plasmid-born copies of the lacI gene exhibited a sensitive and defined response to increasing IPTG concentrations. Photocaged IPTG served as a synthetic photo-switch to convert the E. coli system into an optogenetic expression module allowing for precise and gradual light-triggering of gene expression as demonstrated at the single cell level. |