This title appears in the Scientific Report : 2014 

Light-responsive control of bacterial gene Expression: precise triggering of the lac promoter activity using photocaged IPTG
Binder, Dennis
Grünberger, Alexander / Loeschcke, Anita / Probst, Christopher / Bier, Claus / Pietruszka, Jörg / Wiechert, Wolfgang / Kohlheyer, Dietrich / Jaeger, Karl-Erich (Corresponding Author) / Drepper, Thomas
Biotechnologie; IBG-1
Institut für Bioorganische Chemie (HHUD); IBOC
Integrative biology, 6 (2014) 8, S. 755-765
Cambridge RSC Publ. 2014
10.1039/c4ib00027g
24894989
Journal Article
ohne Topic
OpenAccess
Please use the identifier: http://dx.doi.org/10.1039/c4ib00027g in citations.
Please use the identifier: http://hdl.handle.net/2128/7981 in citations.
Light can be used to control numerous cellular processes including protein function and interaction as well as gene expression in a non-invasive fashion and with unprecedented spatiotemporal resolution. However, for chemical phototriggers tight, gradual, and homogeneous light response has never been attained in living cells. Here, we report on a light-responsive bacterial T7 RNA polymerase expression system based on a photocaged derivative of the inducer molecule isopropyl-β-D-thiogalactopyranoside (IPTG). We have comparatively analyzed different Escherichia coli lac promoter-regulated expression systems in batch and microfluidic single-cell cultivation. The lacY-deficient E. coli strain Tuner(DE3) harboring additional plasmid-born copies of the lacI gene exhibited a sensitive and defined response to increasing IPTG concentrations. Photocaged IPTG served as a synthetic photo-switch to convert the E. coli system into an optogenetic expression module allowing for precise and gradual light-triggering of gene expression as demonstrated at the single cell level.