Transcriptome alterations in zebrafish embryos after exposure to environmental estrogens and anti-androgens can reveal endocrine disruption
Schiller, Viktoria (Corresponding Author)
Wichmann, Arne / Kriehuber, Ralf / Schäfers, Christoph / Fischer, Rainer / Fenske, Martina
Sicherheit und Strahlenschutz, Umgebungsüberwachung,Strahlenbiologie; S-US
Reproductive toxicology, 42 (2013) S. 210 - 223
Amsterdam [u.a.] Elsevier Science 2013
10.1016/j.reprotox.2013.09.003
Journal Article
ohne Topic
Please use the identifier: http://dx.doi.org/10.1016/j.reprotox.2013.09.003 in citations.
Exposure to environmental chemicals known as endocrine disruptors (EDs) is in many cases associated with an unpredictable hazard for wildlife and human health. The identification of endocrine disruptive properties of chemicals certain to enter the aquatic environment relies on toxicity tests with fish, assessing adverse effects on reproduction and sexual development. The demand for quick, reliable ED assays favored the use of fish embryos as alternative test organisms. We investigated the application of a transcriptomics-based assay for estrogenic and anti-androgenic chemicals with zebrafish embryos. Two reference compounds, 17α-ethinylestradiol and flutamide, were tested to evaluate the effects on development and the transcriptome after 48 h-exposures. Comparison of the transcriptome response with other estrogenic and anti-androgenic compounds (genistein, bisphenol A, methylparaben, linuron, prochloraz, propanil) showed commonalities and differences in regulated pathways, enabling us to classify the estrogenic and anti-androgenic potencies. This demonstrates that different mechanism of ED can be assessed already in fish embryos.