This title appears in the Scientific Report :
2014
Please use the identifier:
http://dx.doi.org/10.1016/j.neuropharm.2014.08.022 in citations.
Cockroach GABAB receptor subtypes: Molecular characterization, pharmacological properties and tissue distribution.
Cockroach GABAB receptor subtypes: Molecular characterization, pharmacological properties and tissue distribution.
γ-aminobutyric acid (GABA) is the predominant inhibitory neurotransmitter in the central nervous system (CNS). Its effects are mediated by either ionotropic GABAA receptors or metabotropic GABAB receptors. GABAB receptors regulate, via Gi/o G-proteins, ion channels, and adenylyl cyclases. In humans...
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Personal Name(s): | Blankenburg, S. |
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Balfanz, S. / Hayashi, Y. / Shigenobu, S. / Miura, T. / Baumann, O. / Baumann, A. / Blenau, W. (Corresponding Author) | |
Contributing Institute: |
Zelluläre Biophysik; ICS-4 |
Published in: | Neuropharmacology, 88 (2015) S. 134 - 144 |
Imprint: |
Amsterdam [u.a.]
Elsevier Science
2015
|
PubMed ID: |
25242738 |
DOI: |
10.1016/j.neuropharm.2014.08.022 |
Document Type: |
Journal Article |
Research Program: |
Engineering Cell Function |
Publikationsportal JuSER |
γ-aminobutyric acid (GABA) is the predominant inhibitory neurotransmitter in the central nervous system (CNS). Its effects are mediated by either ionotropic GABAA receptors or metabotropic GABAB receptors. GABAB receptors regulate, via Gi/o G-proteins, ion channels, and adenylyl cyclases. In humans, GABAB receptor subtypes are involved in the etiology of neurologic and psychiatric disorders. In arthropods, however, these members of the G-protein-coupled receptor family are only inadequately characterized. Interestingly, physiological data have revealed important functions of GABAB receptors in the American cockroach, Periplaneta americana. We have cloned cDNAs coding for putative GABAB receptor subtypes 1 and 2 of P. americana (PeaGB1 and PeaGB2). When both receptor proteins are co-expressed in mammalian cells, activation of the receptor heteromer with GABA leads to a dose-dependent decrease in cAMP production. The pharmacological profile differs from that of mammalian and Drosophila GABAB receptors. Western blot analyses with polyclonal antibodies have revealed the expression of PeaGB1 and PeaGB2 in the CNS of the American cockroach. In addition to the widespread distribution in the brain, PeaGB1 is expressed in salivary glands and male accessory glands. Notably, PeaGB1-like immunoreactivity has been detected in the GABAergic salivary neuron 2, suggesting that GABAB receptors act as autoreceptors in this neuron. This article is part of a Special Issue entitled GABAergic signaling. |