Description: Combined Elemental and Biomolecular Mass Spectrometry Imaging for Probing the Inventory of Tissue at a Micrometer Scale

This title appears in the Scientific Report : 2012

Combined Elemental and Biomolecular Mass Spectrometry Imaging for Probing the Inventory of Tissue at a Micrometer Scale

Several complementary mass spectrometric imaging techniques allow mapping of various analytes within biological tissue sections. Laser ablation inductively coupled plasma mass spectrometry (LA-ICPMS) quantitatively detects elements and isotopes with very high sensitivity and a particularly high dyna...

Personal Name(s):	Matusch, A.
	Fenn, L.S. / Depboylu, C. / Klietz, M. / Strohmer, S. / McLean, J.A. / Becker, J.S.
Contributing Institute:	Zentralabteilung für Chemische Analysen; ZCH
Published in:	Analytical chemistry, 84 (2012) S. 3170 - 3178
Imprint:	Columbus, Ohio American Chemical Society 2012
Physical Description:	3170 - 3178
PubMed ID:	22413784
DOI:	10.1021/ac203112c
Document Type:	Journal Article
Research Program:	Theory, modelling and simulation Funktion und Dysfunktion des Nervensystems
Series Title:	Analytical Chemistry 84
Subject (ZB):	Animals Lasers Male Mass Spectrometry: methods Mice Microtechnology: methods Molecular Imaging: methods Optical Processes Oxidopamine: pharmacology Prosencephalon: drug effects Prosencephalon: metabolism Oxidopamine J
	Publikationsportal JuSER

Please use the identifier: http://dx.doi.org/10.1021/ac203112c in citations.

Several complementary mass spectrometric imaging techniques allow mapping of various analytes within biological tissue sections. Laser ablation inductively coupled plasma mass spectrometry (LA-ICPMS) quantitatively detects elements and isotopes with very high sensitivity and a particularly high dynamical range. Matrix-assisted laser desorption/ionization ion mobility mass spectrometry (MALDI-IM-MS) allows a pixel-by-pixel classification and identification of biomolecules. In order to dispose of the healthy hemisphere as an internal calibrant in addition to routinely used external standards, adjacent brain sections of mice with a unilateral 6-OHDA lesion of the medial forebrain bundle were chosen as exemplary samples. We demonstrate a comprehensive way of data acquisition and analysis by coregistering mass spectrometric data on photomicrographs as common reference space and thus providing trimodal spatial information. Registering subsequent planar element maps yielded continuous 3-dimensional data sets. Furthermore, we introduce a correction of MSI data for variable slice thickness applicable to all MSI techniques. In the present case, we observed increased concentrations of iron, manganese, and copper in the lesioned substantia nigra while monounsaturated lipid levels were decreased in the identical region of interest. Our techniques provide new insights into the intricate spatial relationship of morphology and chemistry within tissue.