This title appears in the Scientific Report :
2012
Please use the identifier:
http://dx.doi.org/10.1021/ac203112c in citations.
Combined Elemental and Biomolecular Mass Spectrometry Imaging for Probing the Inventory of Tissue at a Micrometer Scale
Combined Elemental and Biomolecular Mass Spectrometry Imaging for Probing the Inventory of Tissue at a Micrometer Scale
Several complementary mass spectrometric imaging techniques allow mapping of various analytes within biological tissue sections. Laser ablation inductively coupled plasma mass spectrometry (LA-ICPMS) quantitatively detects elements and isotopes with very high sensitivity and a particularly high dyna...
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Personal Name(s): | Matusch, A. |
---|---|
Fenn, L.S. / Depboylu, C. / Klietz, M. / Strohmer, S. / McLean, J.A. / Becker, J.S. | |
Contributing Institute: |
Zentralabteilung für Chemische Analysen; ZCH |
Published in: | Analytical chemistry, 84 (2012) S. 3170 - 3178 |
Imprint: |
Columbus, Ohio
American Chemical Society
2012
|
Physical Description: |
3170 - 3178 |
PubMed ID: |
22413784 |
DOI: |
10.1021/ac203112c |
Document Type: |
Journal Article |
Research Program: |
Theory, modelling and simulation Funktion und Dysfunktion des Nervensystems |
Series Title: |
Analytical Chemistry
84 |
Subject (ZB): | |
Publikationsportal JuSER |
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520 | |a Several complementary mass spectrometric imaging techniques allow mapping of various analytes within biological tissue sections. Laser ablation inductively coupled plasma mass spectrometry (LA-ICPMS) quantitatively detects elements and isotopes with very high sensitivity and a particularly high dynamical range. Matrix-assisted laser desorption/ionization ion mobility mass spectrometry (MALDI-IM-MS) allows a pixel-by-pixel classification and identification of biomolecules. In order to dispose of the healthy hemisphere as an internal calibrant in addition to routinely used external standards, adjacent brain sections of mice with a unilateral 6-OHDA lesion of the medial forebrain bundle were chosen as exemplary samples. We demonstrate a comprehensive way of data acquisition and analysis by coregistering mass spectrometric data on photomicrographs as common reference space and thus providing trimodal spatial information. Registering subsequent planar element maps yielded continuous 3-dimensional data sets. Furthermore, we introduce a correction of MSI data for variable slice thickness applicable to all MSI techniques. In the present case, we observed increased concentrations of iron, manganese, and copper in the lesioned substantia nigra while monounsaturated lipid levels were decreased in the identical region of interest. Our techniques provide new insights into the intricate spatial relationship of morphology and chemistry within tissue. | ||
650 | 2 | |2 MeSH |a Animals | |
650 | 2 | |2 MeSH |a Lasers | |
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650 | 2 | |2 MeSH |a Oxidopamine: pharmacology | |
650 | 2 | |2 MeSH |a Prosencephalon: drug effects | |
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914 | 1 | |y 2012 | |
500 | |a The last author (J.S.B.) would like to acknowledge Hans-Joachim Dietze for fruitful discussion and useful input. We are grateful to Thermo Scientific, Bremen, Germany, and to Deutsche Forschungsgemeinschaft (DFG grant No. BE 2649/5-1) for instrumental support of the Brain Met Laboratory. We thank Astrid Zimmermann for assistance with LA-ICPMS measurements. Martin KH. Schafer and Eberhard Weihe from the Department of Anatomy; Gunter U. Hoglinger, Frauke Neff, and Wolfgang H. Oertel from the Department of Neurology of the Philipps University Marburg, Germany, are thankfully acknowledged. L.S.F., M.K, and JAM would like to acknowledge funding from the National Institutes of Health (RC2DA028981 and 1R01GM092218-01), the US Defense Threat Reduction Agency (HDTRA1-09-1-0013), the Vanderbilt University College of Arts and Sciences, the Vanderbilt Institute of Chemical Biology, and the Vanderbilt Institute for Integrative Biosystems Research and Education. L.S.F was further supported by a VU College of Arts and Sciences Disseration Enhancement Grant. We also thank R. M. Caprioli (VU Biochemistry) and the VUMC Mass Spectrometry Research Core for utilization of the Synapt HDMS. | ||
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