Description: Test-Retest Stability of Cerebral mGluR5 Quantification Using [11C]ABP688 and Positron Emission Tomography in Rats

This title appears in the Scientific Report : 2012

Test-Retest Stability of Cerebral mGluR5 Quantification Using [11C]ABP688 and Positron Emission Tomography in Rats

This study evaluates the reproducibility of the quantification of metabotropic glutamate receptor type 5 (mGluR₅) densities in rats using the PET radiotracer [¹¹C]ABP688 and pharmacokinetic models that are based on an input function, which is derived from a reference tissue. Seven rats underwent dyn...

Personal Name(s):	Elmenhorst, D.
	Aliaga, A. / Bauer, A. / Rosa-Neto, P.
Contributing Institute:	Molekulare Organisation des Gehirns; INM-2
Published in:	Synapse, 66 (2012) S. 552 - 560
Imprint:	New York, NY Wiley-Liss 2012
Physical Description:	552 - 560
DOI:	10.1002/syn.21542
PubMed ID:	22290765
Document Type:	Journal Article
Research Program:	Theory, modelling and simulation Funktion und Dysfunktion des Nervensystems
Series Title:	Synapse 66
Subject (ZB):	Algorithms Animals Brain: radionuclide imaging Brain Chemistry Carbon Radioisotopes: chemistry Image Interpretation, Computer-Assisted Kinetics Male Oximes: analysis Oximes: chemistry Positron-Emission Tomography: methods Pyridines: analysis Pyridines: chemistry Rats Rats, Sprague-Dawley Receptors, Metabotropic Glutamate: analysis Reproducibility of Results 3-(6-methylpyridin-2-ylethynyl)cyclohex-2-enone-O-methyloxime Carbon Radioisotopes Oximes Pyridines Receptors, Metabotropic Glutamate metabotropic glutamate receptor 5 J positron emission tomography test-retest kinetic modeling [11C]ABP688
	Publikationsportal JuSER

Please use the identifier: http://dx.doi.org/10.1002/syn.21542 in citations.

This study evaluates the reproducibility of the quantification of metabotropic glutamate receptor type 5 (mGluR₅) densities in rats using the PET radiotracer [¹¹C]ABP688 and pharmacokinetic models that are based on an input function, which is derived from a reference tissue. Seven rats underwent dynamic PET scans (60 min) after bolus injection of [¹¹C]ABP688. Kinetic analyses included: binding potential (BP(ND) ) determined by calculating (a) the simplified reference tissue model (SRTM) and (b) its two-steps simplified version (SRTM2); (c) multilinear reference tissue model (MRTM) and (d) its 2-parameter version (MRTM2); (e) noninvasive graphical analysis (NIGA). Parametric images were generated representing BP(ND) by the MRTM2 model. BP(ND) determinations were reproducible with low to acceptable variability ranging from 5 to 10% and reproducibility scores (intraclass correlation coefficient) between 0.51 and 0.88. The pharmacokinetic model that showed lowest overall variability was the SRTM. In contrast, the use of the NIGA was associated with significantly lower reproducibility scores. Comparison of parametric images revealed no significant bias between test and retest measurements and is therefore suitable to compare groups at voxel levels. In conclusion, our results suggest that noninvasive quantification of [¹¹C]ABP688 imaging is reproducible and reliable for PET studies of the cerebral mGluR₅ in rats.