This title appears in the Scientific Report :
2015
Please use the identifier:
http://hdl.handle.net/2128/18992 in citations.
Please use the identifier: http://dx.doi.org/10.1063/1.4928512 in citations.
Fast internal dynamics in alcohol dehydrogenase
Fast internal dynamics in alcohol dehydrogenase
Large-scale domain motions in alcohol dehydrogenase (ADH) have been observed previously by neutron spin-echo spectroscopy (NSE). We have extended the investigation on the dynamics of ADH in solution by using high-resolution neutron time-of-flight (TOF) and neutron backscattering (BS) spectroscopy in...
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Personal Name(s): | Monkenbusch, M. (Corresponding author) |
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Stadler, A. / Biehl, R. / Ollivier, J. / Zamponi, M. / Richter, D. | |
Contributing Institute: |
Neutronenstreuung; JCNS-1 JCNS-FRM-II; JCNS-FRM-II Neutronenstreuung; ICS-1 |
Published in: | The journal of chemical physics, 143 (2015) 7, S. 075101 - |
Imprint: |
Melville, NY
American Institute of Physics
2015
|
PubMed ID: |
26298156 |
DOI: |
10.1063/1.4928512 |
Document Type: |
Journal Article |
Research Program: |
Jülich Centre for Neutron Research (JCNS) Soft Matter, Health and Life Sciences Functional Macromolecules and Complexes |
Subject (ZB): | |
Link: |
OpenAccess OpenAccess |
Publikationsportal JuSER |
Please use the identifier: http://dx.doi.org/10.1063/1.4928512 in citations.
Large-scale domain motions in alcohol dehydrogenase (ADH) have been observed previously by neutron spin-echo spectroscopy (NSE). We have extended the investigation on the dynamics of ADH in solution by using high-resolution neutron time-of-flight (TOF) and neutron backscattering (BS) spectroscopy in the incoherent scattering range. The observed hydrogen dynamics were interpreted in terms of three mobility classes, which allowed a simultaneous description of the measured TOF and BS spectra. In addition to the slow global protein diffusion and domain motions observed by NSE, a fast internal process could be identified. Around one third of the protons in ADH participate in the fast localized diffusive motion. The diffusion coefficient of the fast internal motions is around two third of the value of the surrounding D2O solvent. It is tempting to associate the fast internal process with solvent exposed amino acid residues with dangling side chains. |