This title appears in the Scientific Report : 2015 

Nanostructured cavity devices for extracellular stimulation of HL-1 cells
Czeschik, Anna
Rinklin, Philipp / Derra, Ulrike / Ullmann, Sabrina / Holik, Peter / Steltenkamp, Siegfried / Offenhäusser, Andreas / Wolfrum, Bernhard (Corresponding author)
JARA-FIT; JARA-FIT
Bioelektronik; PGI-8
Nanoscale, 7 (2015) 20, S. 9275 - 9281
Cambridge RSC Publ. 2015
10.1039/C5NR01690H
25939765
Journal Article
Engineering Cell Function
OpenAccess
OpenAccess
Please use the identifier: http://dx.doi.org/10.1039/C5NR01690H in citations.
Please use the identifier: http://hdl.handle.net/2128/9682 in citations.
Microelectrode arrays (MEAs) are state-of-the-art devices for extracellular recording and stimulation on biological tissue. Furthermore, they are a relevant tool for the development of biomedical applications like retina, cochlear and motor prostheses, cardiac pacemakers and drug screening. Hence, research on functional cell-sensor interfaces, as well as the development of new surface structures and modifications for improved electrode characteristics, is a vivid and well established field. However, combining single-cell resolution with sufficient signal coupling remains challenging due to poor cell-electrode sealing. Furthermore, electrodes with diameters below 20 µm often suffer from a high electrical impedance affecting the noise during voltage recordings. In this study, we report on a nanocavity sensor array for voltage-controlled stimulation and extracellular action potential recordings on cellular networks. Nanocavity devices combine the advantages of low-impedance electrodes with small cell-chip interfaces, preserving a high spatial resolution for recording and stimulation. A reservoir between opening aperture and electrode is provided, allowing the cell to access the structure for a tight cell-sensor sealing. We present the well-controlled fabrication process and the effect of cavity formation and electrode patterning on the sensor's impedance. Further, we demonstrate reliable voltage-controlled stimulation using nanostructured cavity devices by capturing the pacemaker of an HL-1 cell network.