This title appears in the Scientific Report : 2015 

Optimization of an Enzyme-based Multi-parameter Biosensor for Monitoring Biogas Processes
Pilas, J.
Mariano, K. / Keusgen, M. / Selmer, T. / Schöning, M. J. (Corresponding author)
Bioelektronik; PGI-8
Procedia engineering, 120 (2015) S. 532 - 535
Amsterdam [u.a.] Elsevier 2015
532 - 535
10.1016/j.proeng.2015.08.702
eurosensors 2015, Freiburg (Germany), 2015-09-06 - 2015-09-09
Contribution to a conference proceedings
Journal Article
Controlling Configuration-Based Phenomena
OpenAccess
OpenAccess
Please use the identifier: http://dx.doi.org/10.1016/j.proeng.2015.08.702 in citations.
Please use the identifier: http://hdl.handle.net/2128/9781 in citations.
An enzyme-based multi-parameter sensor chip for the simultaneous measurement of formate, D- and L-lactate is presented. Thereby, the combination of a diaphorase (DIA) from Clostridium kluyveri with different NAD+-dependent dehydrogenases enables the specific recognition of each compound. The amperometric detection is performed via monitoring of the oxidation current of enzymatically produced hexacyanoferrate(II) at +300 mV vs. Ag/AgCl. Chemical cross-linking with glutaraldehyde is used for enzyme immobilization. Thereby, the sensor signal is investigated at different concentrations of the cross-linker in the enzyme membrane. The obtained results indicate that by optimization of the immobilization matrix, the sensor performance can be improved.