This title appears in the Scientific Report : 2015 

Electrostatic Detection of Unlabelled Single- and Double-stranded DNA Using Capacitive Field-effect Devices Functionalized with a Positively Charged Polyelectrolyte Layer
Bronder, T. S.
Poghossian, A. (Corresponding author) / Scheja, S. / Wu, C. S. / Keusgen, M. / Schöning, M. J.
Bioelektronik; PGI-8
Procedia engineering, 120 (2015) S. 544 - 547
Amsterdam [u.a.] Elsevier 2015
544 - 547
Eurosensors 2015, Freiburg (Germany), 2015-09-06 - 2015-09-09
Contribution to a conference proceedings
Journal Article
Controlling Configuration-Based Phenomena
Please use the identifier: in citations.
Please use the identifier: in citations.
Capacitive field-effect electrolyte-insulator-semiconductor sensors consisting of an Al-p-Si-SiO2 structure have been used for the electrical detection of unlabelled single- and double-stranded DNA (dsDNA) molecules by their intrinsic charge. A simple functionalization protocol based on the layer-by-layer (LbL) technique was used to prepare a weak polyelectrolyte/probe-DNA bilayer, followed by the hybridization with complementary target DNA molecules. Due to the flat orientation of the LbL-adsorbed DNA molecules, a high sensor signal has been achieved. In addition, direct label-free detection of in-solution hybridized dsDNA molecules has been studied.