This title appears in the Scientific Report :
2015
Please use the identifier:
http://dx.doi.org/10.1016/j.proeng.2015.08.710 in citations.
Please use the identifier: http://hdl.handle.net/2128/9783 in citations.
Electrostatic Detection of Unlabelled Single- and Double-stranded DNA Using Capacitive Field-effect Devices Functionalized with a Positively Charged Polyelectrolyte Layer
Electrostatic Detection of Unlabelled Single- and Double-stranded DNA Using Capacitive Field-effect Devices Functionalized with a Positively Charged Polyelectrolyte Layer
Capacitive field-effect electrolyte-insulator-semiconductor sensors consisting of an Al-p-Si-SiO2 structure have been used for the electrical detection of unlabelled single- and double-stranded DNA (dsDNA) molecules by their intrinsic charge. A simple functionalization protocol based on the layer-by...
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Personal Name(s): | Bronder, T. S. |
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Poghossian, A. (Corresponding author) / Scheja, S. / Wu, C. S. / Keusgen, M. / Schöning, M. J. | |
Contributing Institute: |
Bioelektronik; PGI-8 |
Published in: | Procedia engineering, 120 (2015) S. 544 - 547 |
Imprint: |
Amsterdam [u.a.]
Elsevier
2015
|
Physical Description: |
544 - 547 |
DOI: |
10.1016/j.proeng.2015.08.710 |
Conference: | Eurosensors 2015, Freiburg (Germany), 2015-09-06 - 2015-09-09 |
Document Type: |
Contribution to a conference proceedings Journal Article |
Research Program: |
Controlling Configuration-Based Phenomena |
Link: |
OpenAccess OpenAccess |
Publikationsportal JuSER |
Please use the identifier: http://hdl.handle.net/2128/9783 in citations.
Capacitive field-effect electrolyte-insulator-semiconductor sensors consisting of an Al-p-Si-SiO2 structure have been used for the electrical detection of unlabelled single- and double-stranded DNA (dsDNA) molecules by their intrinsic charge. A simple functionalization protocol based on the layer-by-layer (LbL) technique was used to prepare a weak polyelectrolyte/probe-DNA bilayer, followed by the hybridization with complementary target DNA molecules. Due to the flat orientation of the LbL-adsorbed DNA molecules, a high sensor signal has been achieved. In addition, direct label-free detection of in-solution hybridized dsDNA molecules has been studied. |