This title appears in the Scientific Report :
2016
Please use the identifier:
http://dx.doi.org/10.1002/cbic.201500609 in citations.
Photocaged Arabinose - A Novel Optogenetic Switch for Rapid and Gradual Control of Microbial Gene Expression
Photocaged Arabinose - A Novel Optogenetic Switch for Rapid and Gradual Control of Microbial Gene Expression
Controlling cellular functions by light allows simple triggering of biological processes in a non-invasive fashion with high spatiotemporal resolution. In this context, light-regulated gene expression has enormous potential for achieving optogenetic control over almost any cellular process. Here, we...
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Personal Name(s): | Binder, Dennis |
---|---|
Bier, Claus / Grünberger, Alexander / Drobietz, Dagmar / Hage-Hülsmann, Jennifer / Wandrey, Georg / Büchs, Jochen / Kohlheyer, Dietrich / Loeschcke, Anita / Wiechert, Wolfgang / Jaeger, Karl-Erich / Pietruszka, Jörg / Drepper, Thomas (Corresponding author) | |
Contributing Institute: |
Institut für Molekulare Enzymtechnologie (HHUD); IMET Biotechnologie; IBG-1 Institut für Bioorganische Chemie (HHUD); IBOC |
Published in: | ChemBioChem, 17 (2016) 4, S. 296-299 |
Imprint: |
Weinheim
Wiley-VCH
2016
|
DOI: |
10.1002/cbic.201500609 |
PubMed ID: |
26677142 |
Document Type: |
Journal Article |
Research Program: |
Biotechnology |
Publikationsportal JuSER |
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520 | |a Controlling cellular functions by light allows simple triggering of biological processes in a non-invasive fashion with high spatiotemporal resolution. In this context, light-regulated gene expression has enormous potential for achieving optogenetic control over almost any cellular process. Here, we report on two novel one-step cleavable photocaged arabinose compounds, which were applied as light-sensitive inducers of transcription in bacteria. Exposure of caged arabinose to UV-A light resulted in rapid activation of protein production, as demonstrated for GFP and the complete violacein biosynthetic pathway. Moreover, single-cell analysis revealed that intrinsic heterogeneity of arabinose-mediated induction of gene expression was overcome when using photocaged arabinose. We have thus established a novel phototrigger for synthetic bio(techno)logy applications that enables precise and homogeneous control of bacterial target gene expression. | ||
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