This title appears in the Scientific Report : 2016 

Preparation and Crystallization of Perdeuterated T4 Phage Lysozyme for Neutron Diffraction Study
Hiromoto, Takeshi (Corresponding author)
Adachi, Motoyasu / Shibazaki, Chie / Schrader, Tobias E. / Ostermann, Andreas / Kuroki, Ryota
Neutronenstreuung; JCNS-1
JCNS-FRM-II; JCNS-FRM-II
JPS conference proceedings, 8 (2015) S. 033003
Tokyo The Physical Society of Japan 2015
10.7566/JPSCP.8.033003
Proceedings of the 2nd International Symposium on Science at J-PARC — Unlocking the Mysteries of Life, Matter and the Universe —, Tsukuba (Ibaraki),
Journal Article
Jülich Centre for Neutron Research (JCNS)
FRM II / MLZ
Please use the identifier: http://dx.doi.org/10.7566/JPSCP.8.033003 in citations.
T4 phage lysozyme (T4L) is an endoacetylmuramidase that degrades the murein of the bacterial cell wall by cleaving the β-1,4-glycosidic bond between N-acetylmuramic acid and N-acetylglucosamine. We previously reported that the substitution of the catalytic Thr26 with the nucleophilic His converts the wild-type (WT) T4L from an inverting to a retaining glycosidase, in which the β-configuration of the substrate is retained in the product. We also found that the Thr26His (T26H) mutant can catalyze a transglycosylation reaction more effectively than hydrolysis, although the WT-T4L has no transglycosidase activity. To clarify the role of the substituted His26 in transglycosylation and to investigate its relationship to the neighboring acidic residue Asp20 using neutron crystallography, a perdeuterated recombinant protein of the T26H mutant (d-T26H) was prepared for crystallization. The perdeuteration would reduce the crystal-size requirement by one order of magnitude and enable better visualization of the protonation and hydration states of the catalytic residues. The perdeuterated form was produced in Escherichia coli cells cultured in deuterated-rich media. After purification, the d-T26H mutant was crystallized under deuterated conditions and grown to a volume of 0.12 mm3 using a macroseeding technique. A preliminary neutron-diffraction experiment at 100 K at the FRM II research reactor (Munich, Germany) gave diffraction spots of up to 2.8 Å resolution after a 1.5 h exposure.