This title appears in the Scientific Report :
2003
Please use the identifier:
http://dx.doi.org/10.1016/S0956-5663(02)00178-1 in citations.
Flow injection amperometric detection of OP nerve agents based on organophosphorus-hydrolase biosensor detector
Flow injection amperometric detection of OP nerve agents based on organophosphorus-hydrolase biosensor detector
A flow-injection system with an organophosphorus-hydrolase (OPH)-biosensor detector has been developed and characterized for the rapid detection of organophosphorus (OP) nerve agents. The enzyme was immobilized onto a thin-film gold detector through a cystamine-glutaraldehyde coupling. Factors influ...
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Personal Name(s): | Wang, J. |
---|---|
Krause, R. / Block, K. / Musameh, M. / Mulchandani, A. / Schöning, M. J. | |
Contributing Institute: |
Institut für Bio- und Chemosensoren; ISG-2 |
Published in: | Biosensors and bioelectronics, 18 (2003) S. 255 - 260 |
Imprint: |
Amsterdam [u.a.]
Elsevier Science
2003
|
Physical Description: |
255 - 260 |
DOI: |
10.1016/S0956-5663(02)00178-1 |
Document Type: |
Journal Article |
Research Program: |
Materialien, Prozesse und Bauelemente für die Mikro- und Nanoelektronik |
Subject (ZB): | |
Publikationsportal JuSER |
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520 | |a A flow-injection system with an organophosphorus-hydrolase (OPH)-biosensor detector has been developed and characterized for the rapid detection of organophosphorus (OP) nerve agents. The enzyme was immobilized onto a thin-film gold detector through a cystamine-glutaraldehyde coupling. Factors influencing the performance were optimized. The resulting flow system offered a fast, sensitive, selective, and stable response. The peak current increased linearly with the concentration of paraoxon and methyl parathion over the 1-10 muM range (sensitivity, 2.29 and 1.04 nA/muM, respectively). The OPH-biosensor flow injection systems offered low detection limits (e.g. 0.1 muM paraoxon), along with a good precision (R.S.D. of 3.6% for 20 successive injections of a 1.0 muM paraoxon solution). The OPH-biosensor flow detector offers great promise for rapid field screening of OP pesticides and nerve agents. (C) 2002 Elsevier Science B.V. All rights reserved. | ||
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