This title appears in the Scientific Report : 2003 

Biological effects induced by swift heavy ions of Lithium on aqueous solution of plasmid pMTa4
Humtsoe, J. O.
Schneeweiss, F. H. A. / Srivastava, A. / Sarma, A. / Sharan, R. N.
Institut für Medizin; IME
Abteilung Sicherheit und Strahlenschutz; S
Radiation effects and defects in solids, 158 (2003) S. 603 - 607
London [u.a.] Taylor & Francis 2003
603 - 607
10.1080/1042015031000116096
Journal Article
Nukleare Sicherheitsforschung
Neurowissenschaften
Radiation Effects and Defects in Solids 158
J
Please use the identifier: http://dx.doi.org/10.1080/1042015031000116096 in citations.
Biological samples exposed to swift heavy ions sustain damage on different components. Damage to DNA, a critical component of a living system, has considerable biological implications. In this study aqueous solution of plasmid pMTa4 was exposed to varying fluence of swift Li-7 ions and its different topological forms were analysed by agarose gel electrophoresis to study the induced damage. To monitor radiation labile nucleotide sequence the Li-7 ions exposed plasmid was degraded by three different restriction endonucleases and also analysed by agarose gel electrophoresis. The results show that Li-7 ions predominantly induced double strand breaks in the plasmid DNA in a dose-dependent manner and affected preferentially the GC-rich motifs of the DNA. The results suggest that Li-7 ions induce premutagenic lesions at an enhanced frequency in segments of the DNA which are rich in CG content as compared to GC-poor segments.