This title appears in the Scientific Report :
2006
Please use the identifier:
http://hdl.handle.net/2128/796 in citations.
Please use the identifier: http://dx.doi.org/10.1128/AEM.72.2.1688-1691.2006 in citations.
Improvement of Oligonucleotide Probe Design Criteria for Functional Gene Microarrays in Environmental applications
Improvement of Oligonucleotide Probe Design Criteria for Functional Gene Microarrays in Environmental applications
To optimize oligonucleotide probe design criteria, PCR products with different similarities to probes were hybridized to a functional gene microarray designed to detect homologous genes from different organisms. In contrast to more restrictive probe designs based on a single criterion, simultaneous...
Saved in:
Personal Name(s): | Liebich, J. |
---|---|
Schadt, C. W. / Chong, S. C. / He, Z. / Rhee, S.-K. / Zhou, J.-H. | |
Contributing Institute: |
Agrosphäre; ICG-IV |
Published in: | Applied and environmental microbiology, 72 (2006) S. 1688 - 1691 |
Imprint: |
Washington, DC [u.a.]
Soc.
2006
|
Physical Description: |
1688 - 1691 |
DOI: |
10.1128/AEM.72.2.1688-1691.2006 |
PubMed ID: |
16461729 |
Document Type: |
Journal Article |
Research Program: |
Terrestrische Umwelt |
Series Title: |
Applied and Environmental Microbiology
72 |
Subject (ZB): | |
Link: |
Get full text OpenAccess |
Publikationsportal JuSER |
Please use the identifier: http://dx.doi.org/10.1128/AEM.72.2.1688-1691.2006 in citations.
To optimize oligonucleotide probe design criteria, PCR products with different similarities to probes were hybridized to a functional gene microarray designed to detect homologous genes from different organisms. In contrast to more restrictive probe designs based on a single criterion, simultaneous consideration of the percent similarity (< or =90%), the length of identical sequence stretches (< or =20 bases), and the binding free energy (> or =-35 kcal mol(-1)) was found to be predictive of probe specificity. |