This title appears in the Scientific Report :
2006
Please use the identifier:
http://dx.doi.org/10.1002/biot.200600032 in citations.
Modification of the membrane-bound glucose oxidation system in Gluconobacter oxydans significantly increases gluconate and 5-keto-D-gluconic acid accumulation
Modification of the membrane-bound glucose oxidation system in Gluconobacter oxydans significantly increases gluconate and 5-keto-D-gluconic acid accumulation
Gluconobacter oxydans DSM 2343 (ATCC 621H)catalyzes the oxidation of glucose to gluconic acid and subsequently to 5-keto-D-gluconic acid (5-KGA), a precursor of the industrially important L-(+)-tartaric acid. To further increase 5-KGA production in G. oxydans, the mutant strain MF1 was used. In this...
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Personal Name(s): | Merfort, M. |
---|---|
Herrmann, U. / Ha, S.-W. / Elfari, M. / Bringer-Meyer, S. / Görisch, H. / Sahm, H. | |
Contributing Institute: |
Biotechnologie 1; IBT-1 |
Published in: | Biotechnology journal, 1 (2006) S. 556 - 563 |
Imprint: |
Weinheim
Wiley-VCH
2006
|
Physical Description: |
556 - 563 |
PubMed ID: |
16892291 |
DOI: |
10.1002/biot.200600032 |
Document Type: |
Journal Article |
Research Program: |
Biotechnologie |
Series Title: |
Biotechnology Journal
1 |
Subject (ZB): | |
Publikationsportal JuSER |
Gluconobacter oxydans DSM 2343 (ATCC 621H)catalyzes the oxidation of glucose to gluconic acid and subsequently to 5-keto-D-gluconic acid (5-KGA), a precursor of the industrially important L-(+)-tartaric acid. To further increase 5-KGA production in G. oxydans, the mutant strain MF1 was used. In this strain the membrane-bound gluconate-2-dehydrogenase activity, responsible for formation of the undesired by-product 2-keto-D-gluconic acid, is disrupted. Therefore, high amounts of 5-KGA accumulate in the culture medium. G. oxydans MF1 was equipped with plasmids allowing the overexpression of the membrane-bound enzymes involved in 5-KGA formation. Overexpression was confirmed on the transcript and enzymatic level. Furthermore, the resulting strains overproducing the membrane-bound glucose dehydrogenase showed an increased gluconic acid formation, whereas the overproduction of gluconate-5-dehydrogenase resulted in an increase in 5-KGA of up to 230 mM. Therefore, these newly developed recombinant strains provide a basis for further improving the biotransformation process for 5-KGA production. |