Description: Challenge by the murine brain: Multi-pinhole SPECT of 123I-labelled pharmaceuticals

This title appears in the Scientific Report : 2008

Challenge by the murine brain: Multi-pinhole SPECT of 123I-labelled pharmaceuticals

This protocol presents an improved method for SPECT imaging based on multi-pinhole techniques, applied to the visualisation of neurotracers in small animal models. Three types of collimators with 6-pinhole apertures adapted to special requirements for the imaging of the brain of mice and rats and to...

Personal Name(s):	Pissarek, M.B.
	Oros-Peusquens, A.-M. / Schramm, N.U.
Contributing Institute:	Institut für Neurowissenschaften und Biophysik - Medizin; INB-3 Zentralinstitut für Elektronik; ZEL Nuklearchemie; INB-4
Published in:	Journal of neuroscience methods, 168 (2008) S. 282 - 292
Imprint:	Amsterdam [u.a.] Elsevier Science 2008
Physical Description:	282 - 292
DOI:	10.1016/j.jneumeth.2007.10.011
PubMed ID:	18061274
Document Type:	Journal Article
Research Program:	Funktion und Dysfunktion des Nervensystems
Series Title:	Journal of Neuroscience Methods 168
Subject (ZB):	Anesthesia Animals Benzamides: diagnostic use Brain: radionuclide imaging Iodine Radioisotopes: diagnostic use Magnetic Resonance Imaging Mice Organotechnetium Compounds: diagnostic use Pyrrolidines: diagnostic use Radiopharmaceuticals: diagnostic use Radiopharmaceuticals: pharmacokinetics Rats Rats, Inbred WKY Reference Standards Technetium Tc 99m Exametazime: diagnostic use Thyroid Gland: metabolism Tomography, Emission-Computed, Single-Photon: methods Tropanes: diagnostic use Benzamides Iodine Radioisotopes Organotechnetium Compounds Pyrrolidines Radiopharmaceuticals Tropanes technetium Tc 99m TRODAT-1 Technetium Tc 99m Exametazime 2-carbomethoxy-8-(3-fluoropropyl)-3-(4-iodophenyl)tropane 3-iodo-2-hydroxy-6-methoxy-N-((1-ethyl-2-pyrrolidinyl)methyl)benzamide J brain dopamine transporter dopamine receptor [I-123]FP-CIT [I-123]IBZM mouse MRI multi-pinhole SPECT
	Publikationsportal JuSER

Please use the identifier: http://dx.doi.org/10.1016/j.jneumeth.2007.10.011 in citations.

This protocol presents an improved method for SPECT imaging based on multi-pinhole techniques, applied to the visualisation of neurotracers in small animal models. Three types of collimators with 6-pinhole apertures adapted to special requirements for the imaging of the brain of mice and rats and to full body imaging in mice are employed in the experiments. A conventional triple-headed TRIAD/Trionix SPECT system was upgraded with pyramidal supports and shieldings onto the multi-pinhole collimators were installed. The system was employed for the assessment of the uptake of [123I]FP-CIT and [123I]IBZM, well known tracers of dopamine transport and dopamine D2/D3 receptors, respectively. Requirements regarding the applied radioactivity are reported, as well as further conditions determining the effectiveness of the detection of the uptake of [123I]FP-CIT and [123I]IBZM. The measurements in mice required only 20-25% of the activity described in previous studies. Dynamic measurements are presented, with a time resolution as high as 10 min in the brain of rats. Due to the lower signal intensity obtained for mice, the time resolution was 42min for [123I]FP-CIT, with a ratio ROI/background of 5.4, and 17 min for [123I]IBZM, with the ratio ROI/background of 4.5 (1.6-7.4).