This title appears in the Scientific Report :
2008
Please use the identifier:
http://dx.doi.org/10.1016/j.jneumeth.2007.10.011 in citations.
Challenge by the murine brain: Multi-pinhole SPECT of 123I-labelled pharmaceuticals
Challenge by the murine brain: Multi-pinhole SPECT of 123I-labelled pharmaceuticals
This protocol presents an improved method for SPECT imaging based on multi-pinhole techniques, applied to the visualisation of neurotracers in small animal models. Three types of collimators with 6-pinhole apertures adapted to special requirements for the imaging of the brain of mice and rats and to...
| Personal Name(s): | Pissarek, M.B. |
|---|---|
| Oros-Peusquens, A.-M. / Schramm, N.U. | |
| Contributing Institute: |
Institut für Neurowissenschaften und Biophysik - Medizin; INB-3 Zentralinstitut für Elektronik; ZEL Nuklearchemie; INB-4 |
| Published in: | Journal of neuroscience methods, 168 (2008) S. 282 - 292 |
| Imprint: |
Amsterdam [u.a.]
Elsevier Science
2008
|
| Physical Description: |
282 - 292 |
| DOI: |
10.1016/j.jneumeth.2007.10.011 |
| PubMed ID: |
18061274 |
| Document Type: |
Journal Article |
| Research Program: |
Funktion und Dysfunktion des Nervensystems |
| Series Title: |
Journal of Neuroscience Methods
168 |
| Subject (ZB): | |
| Publikationsportal JuSER |
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| 084 | |2 WoS |a Neurosciences | ||
| 100 | 1 | |a Pissarek, M.B. |b 0 |u FZJ |0 P:(DE-Juel1)VDB73461 | |
| 245 | |a Challenge by the murine brain: Multi-pinhole SPECT of 123I-labelled pharmaceuticals | ||
| 260 | |a Amsterdam [u.a.] |b Elsevier Science |c 2008 | ||
| 300 | |a 282 - 292 | ||
| 440 | 0 | |a Journal of Neuroscience Methods |x 0165-0270 |0 9911 |y 2 |v 168 | |
| 500 | |a Record converted from VDB: 12.11.2012 | ||
| 520 | |a This protocol presents an improved method for SPECT imaging based on multi-pinhole techniques, applied to the visualisation of neurotracers in small animal models. Three types of collimators with 6-pinhole apertures adapted to special requirements for the imaging of the brain of mice and rats and to full body imaging in mice are employed in the experiments. A conventional triple-headed TRIAD/Trionix SPECT system was upgraded with pyramidal supports and shieldings onto the multi-pinhole collimators were installed. The system was employed for the assessment of the uptake of [123I]FP-CIT and [123I]IBZM, well known tracers of dopamine transport and dopamine D2/D3 receptors, respectively. Requirements regarding the applied radioactivity are reported, as well as further conditions determining the effectiveness of the detection of the uptake of [123I]FP-CIT and [123I]IBZM. The measurements in mice required only 20-25% of the activity described in previous studies. Dynamic measurements are presented, with a time resolution as high as 10 min in the brain of rats. Due to the lower signal intensity obtained for mice, the time resolution was 42min for [123I]FP-CIT, with a ratio ROI/background of 5.4, and 17 min for [123I]IBZM, with the ratio ROI/background of 4.5 (1.6-7.4). | ||
| 588 | |a Dataset connected to Web of Science, Pubmed | ||
| 650 | 2 | |2 MeSH |a Anesthesia | |
| 650 | 2 | |2 MeSH |a Animals | |
| 650 | 2 | |2 MeSH |a Benzamides: diagnostic use | |
| 650 | 2 | |2 MeSH |a Brain: radionuclide imaging | |
| 650 | 2 | |2 MeSH |a Iodine Radioisotopes: diagnostic use | |
| 650 | 2 | |2 MeSH |a Magnetic Resonance Imaging | |
| 650 | 2 | |2 MeSH |a Mice | |
| 650 | 2 | |2 MeSH |a Organotechnetium Compounds: diagnostic use | |
| 650 | 2 | |2 MeSH |a Pyrrolidines: diagnostic use | |
| 650 | 2 | |2 MeSH |a Radiopharmaceuticals: diagnostic use | |
| 650 | 2 | |2 MeSH |a Radiopharmaceuticals: pharmacokinetics | |
| 650 | 2 | |2 MeSH |a Rats | |
| 650 | 2 | |2 MeSH |a Rats, Inbred WKY | |
| 650 | 2 | |2 MeSH |a Reference Standards | |
| 650 | 2 | |2 MeSH |a Technetium Tc 99m Exametazime: diagnostic use | |
| 650 | 2 | |2 MeSH |a Thyroid Gland: metabolism | |
| 650 | 2 | |2 MeSH |a Tomography, Emission-Computed, Single-Photon: methods | |
| 650 | 2 | |2 MeSH |a Tropanes: diagnostic use | |
| 650 | 7 | |0 0 |2 NLM Chemicals |a Benzamides | |
| 650 | 7 | |0 0 |2 NLM Chemicals |a Iodine Radioisotopes | |
| 650 | 7 | |0 0 |2 NLM Chemicals |a Organotechnetium Compounds | |
| 650 | 7 | |0 0 |2 NLM Chemicals |a Pyrrolidines | |
| 650 | 7 | |0 0 |2 NLM Chemicals |a Radiopharmaceuticals | |
| 650 | 7 | |0 0 |2 NLM Chemicals |a Tropanes | |
| 650 | 7 | |0 0 |2 NLM Chemicals |a technetium Tc 99m TRODAT-1 | |
| 650 | 7 | |0 100504-35-6 |2 NLM Chemicals |a Technetium Tc 99m Exametazime | |
| 650 | 7 | |0 155797-99-2 |2 NLM Chemicals |a 2-carbomethoxy-8-(3-fluoropropyl)-3-(4-iodophenyl)tropane | |
| 650 | 7 | |0 84226-06-2 |2 NLM Chemicals |a 3-iodo-2-hydroxy-6-methoxy-N-((1-ethyl-2-pyrrolidinyl)methyl)benzamide | |
| 650 | 7 | |a J |2 WoSType | |
| 653 | 2 | 0 | |2 Author |a brain |
| 653 | 2 | 0 | |2 Author |a dopamine transporter |
| 653 | 2 | 0 | |2 Author |a dopamine receptor |
| 653 | 2 | 0 | |2 Author |a [I-123]FP-CIT |
| 653 | 2 | 0 | |2 Author |a [I-123]IBZM |
| 653 | 2 | 0 | |2 Author |a mouse |
| 653 | 2 | 0 | |2 Author |a MRI |
| 653 | 2 | 0 | |2 Author |a multi-pinhole SPECT |
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