Description: Targeting murine heart and brain: visualisation conditions for multi-pinhole SPECT with 99mTc- and 123I-labelled probes

This title appears in the Scientific Report : 2009

Targeting murine heart and brain: visualisation conditions for multi-pinhole SPECT with 99mTc- and 123I-labelled probes

The study serves to optimise conditions for multi-pinhole SPECT small animal imaging of (123)I- and (99m)Tc-labelled radiopharmaceuticals with different distributions in murine heart and brain and to investigate detection and dose range thresholds for verification of differences in tracer uptake.A T...

Personal Name(s):	Pissarek, M.
	Meyer-Kirchrath, J. / Hohlfeld, T. / Vollmar, S. / Oros-Peusquens, A.M. / Flögel, U. / Jacoby, C. / Krügel, U. / Schramm, N.
Contributing Institute:	Physik der Medizinischen Bildgebung; INM-4 Zentralinstitut für Elektronik; ZEL Nuklearchemie; INM-5
Published in:	European Journal of Nuclear Medicine and Molecular Imaging, 36 (2009) S. 1495 - 1509
Imprint:	Heidelberg [u.a.] Springer-Verl. 2009
Physical Description:	1495 - 1509
PubMed ID:	19421750
DOI:	10.1007/s00259-009-1142-9
Document Type:	Journal Article
Research Program:	Funktion und Dysfunktion des Nervensystems
Series Title:	European Journal of Nuclear Medicine and Molecular Imaging 36
Subject (ZB):	Animals Benzamides: pharmacokinetics Brain: metabolism Brain: radionuclide imaging Cardiomyopathies: metabolism Cardiomyopathies: radionuclide imaging Heart: radionuclide imaging Humans Iodine Radioisotopes Iodobenzenes: pharmacokinetics Mice Mice, Transgenic Myocardium: metabolism Oximes: pharmacokinetics Pyrrolidines: pharmacokinetics Radiopharmaceuticals: pharmacokinetics Receptors, Prostaglandin E: metabolism Receptors, Prostaglandin E, EP3 Subtype Swine Technetium Technetium Tc 99m Sestamibi: pharmacokinetics Tomography, Emission-Computed, Single-Photon Benzamides Iodobenzenes Oximes PTGER3 protein, human Ptger3 protein, mouse Pyrrolidines Radiopharmaceuticals Receptors, Prostaglandin E hexamethylpropyleneamine oxime Technetium Tc 99m Sestamibi omega-(4-iodophenyl)pentadecanoic acid 3-iodo-2-hydroxy-6-methoxy-N-((1-ethyl-2-pyrrolidinyl)methyl)benzamide J Brain Heart [I-123]IBZM Multi-pinhole SPECT [Tc-99m]sestamibi
	Publikationsportal JuSER

Please use the identifier: http://dx.doi.org/10.1007/s00259-009-1142-9 in citations.

The study serves to optimise conditions for multi-pinhole SPECT small animal imaging of (123)I- and (99m)Tc-labelled radiopharmaceuticals with different distributions in murine heart and brain and to investigate detection and dose range thresholds for verification of differences in tracer uptake.A Triad 88/Trionix system with three 6-pinhole collimators was used for investigation of dose requirements for imaging of the dopamine D(2) receptor ligand [(123)I]IBZM and the cerebral perfusion tracer [(99m)Tc]HMPAO (1.2-0.4 MBq/g body weight) in healthy mice. The fatty acid [(123)I]IPPA (0.94 +/- 0.05 MBq/g body weight) and the perfusion tracer [(99m)Tc]sestamibi (3.8 +/- 0.45 MBq/g body weight) were applied to cardiomyopathic mice overexpressing the prostaglandin EP(3) receptor.In vivo imaging and in vitro data revealed 45 kBq total cerebral uptake and 201 kBq cardiac uptake as thresholds for visualisation of striatal [(123)I]IBZM and of cardiac [(99m)Tc]sestamibi using 100 and 150 s acquisition time, respectively. Alterations of maximal cerebral uptake of [(123)I]IBZM by >20% (116 kBq) were verified with the prerequisite of 50% striatal of total uptake. The labelling with [(99m)Tc]sestamibi revealed a 30% lower uptake in cardiomyopathic hearts compared to wild types. [(123)I]IPPA uptake could be visualised at activity doses of 0.8 MBq/g body weight.Multi-pinhole SPECT enables detection of alterations of the cerebral uptake of (123)I- and (99m)Tc-labelled tracers in an appropriate dose range in murine models targeting physiological processes in brain and heart. The thresholds of detection for differences in the tracer uptake determined under the conditions of our experiments well reflect distinctions in molar activity and uptake characteristics of the tracers.