This title appears in the Scientific Report :
2008
Please use the identifier:
http://dx.doi.org/10.1529/biophysj.108.138040 in citations.
Hemoglobin Dynamics in Red Blood Cells: Correlation to Body Temperature
Hemoglobin Dynamics in Red Blood Cells: Correlation to Body Temperature
A transition in hemoglobin behavior at close to body temperature has been discovered recently by micropipette aspiration experiments on single red blood cells (RBCs) and circular dichroism spectroscopy on hemoglobin solutions. The transition temperature was directly correlated to the body temperatur...
Saved in:
Personal Name(s): | Stadler, A.M. |
---|---|
Digel, I. / Artmann, G.M. / Embs, J.P. / Zaccai, G. / Büldt, G. | |
Contributing Institute: |
Molekulare Biophysik; INB-2 |
Published in: | Biophysical journal, 95 (2008) S. 5449 - 5461 |
Imprint: |
New York, NY
Rockefeller Univ. Press
2008
|
Physical Description: |
5449 - 5461 |
DOI: |
10.1529/biophysj.108.138040 |
PubMed ID: |
18708462 |
Document Type: |
Journal Article |
Research Program: |
Funktion und Dysfunktion des Nervensystems |
Series Title: |
Biophysical Journal
95 |
Subject (ZB): | |
Publikationsportal JuSER |
LEADER | 04422nam a2200781 a 4500 | ||
---|---|---|---|
001 | 763 | ||
005 | 20200402205414.0 | ||
700 | 1 | |0 P:(DE-HGF)0 |a Digel, I. |b 1 | |
700 | 1 | |0 P:(DE-HGF)0 |a Artmann, G.M. |b 2 | |
700 | 1 | |0 P:(DE-HGF)0 |a Embs, J.P. |b 3 | |
700 | 1 | |0 P:(DE-HGF)0 |a Zaccai, G. |b 4 | |
700 | 1 | |0 P:(DE-Juel1)131957 |a Büldt, G. |b 5 |u FZJ | |
856 | 7 | |2 Pubmed Central |u http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2586580 | |
909 | C | O | |o oai:juser.fz-juelich.de:763 |p VDB |
970 | |a VDB:(DE-Juel1)101420 | ||
980 | |a VDB | ||
980 | |a ConvertedRecord | ||
980 | |a journal | ||
980 | |a I:(DE-Juel1)ISB-2-20090406 | ||
980 | |a UNRESTRICTED | ||
980 | |a I:(DE-Juel1)ICS-6-20110106 | ||
520 | |a A transition in hemoglobin behavior at close to body temperature has been discovered recently by micropipette aspiration experiments on single red blood cells (RBCs) and circular dichroism spectroscopy on hemoglobin solutions. The transition temperature was directly correlated to the body temperatures of a variety of species. In an exploration of the molecular basis for the transition, we present neutron scattering measurements of the temperature dependence of hemoglobin dynamics in whole human RBCs in vivo. The data reveal a change in the geometry of internal protein motions at 36.9 degrees C, at human body temperature. Above that temperature, amino acid side-chain motions occupy larger volumes than expected from normal temperature dependence, indicating partial unfolding of the protein. Global protein diffusion in RBCs was also measured and the findings compared favorably with theoretical predictions for short-time self-diffusion of noncharged hard-sphere colloids. The results demonstrated that changes in molecular dynamics in the picosecond time range and angstrom length scale might well be connected to a macroscopic effect on whole RBCs that occurs at body temperature. | ||
650 | 2 | |2 MeSH |a Body Temperature | |
650 | 2 | |2 MeSH |a Diffusion | |
650 | 2 | |2 MeSH |a Elasticity | |
650 | 2 | |2 MeSH |a Erythrocytes: metabolism | |
650 | 2 | |2 MeSH |a Hemoglobins: metabolism | |
650 | 2 | |2 MeSH |a Humans | |
650 | 2 | |2 MeSH |a Neutron Diffraction | |
650 | 2 | |2 MeSH |a Protein Denaturation | |
650 | 7 | |0 0 |2 NLM Chemicals |a Hemoglobins | |
650 | 7 | |2 WoSType |a J | |
915 | |0 StatID:(DE-HGF)0010 |a JCR/ISI refereed | ||
914 | 1 | |y 2008 | |
500 | |a This research project was supported by the European Commission under the 6th Framework Programme through Key Action: Strengthening the European Research Area, Research Infrastructures, contract No. RII3-CT-2003505925. | ||
773 | |0 PERI:(DE-600)1477214-0 |a 10.1529/biophysj.108.138040 |g Vol. 95, p. 5449 - 5461 |p 5449 - 5461 |q 95<5449 - 5461 |t Biophysical journal |v 95 |x 0006-3495 |y 2008 | ||
300 | |a 5449 - 5461 | ||
082 | |a 570 | ||
440 | 0 | |0 882 |a Biophysical Journal |v 95 |x 0006-3495 |y 11 | |
588 | |a Dataset connected to Web of Science, Pubmed | ||
084 | |2 WoS |a Biophysics | ||
245 | |a Hemoglobin Dynamics in Red Blood Cells: Correlation to Body Temperature | ||
024 | 7 | |2 pmid |a pmid:18708462 | |
024 | 7 | |2 pmc |a pmc:PMC2586580 | |
024 | 7 | |2 DOI |a 10.1529/biophysj.108.138040 | |
024 | 7 | |2 WOS |a WOS:000260999500043 | |
037 | |a PreJuSER-763 | ||
260 | |a New York, NY |b Rockefeller Univ. Press |c 2008 | ||
100 | 1 | |0 P:(DE-Juel1)VDB78506 |a Stadler, A.M. |b 0 |u FZJ | |
041 | |a eng | ||
913 | 1 | |0 G:(DE-Juel1)FUEK409 |a DE-HGF |b Gesundheit |k P33 |l Funktion und Dysfunktion des Nervensystems |v Funktion und Dysfunktion des Nervensystems |x 0 | |
536 | |a Funktion und Dysfunktion des Nervensystems |0 G:(DE-Juel1)FUEK409 |2 G:(DE-HGF) |c P33 |x 0 | ||
336 | |a ARTICLE |2 BibTeX | ||
336 | |a Nanopartikel unedler Metalle (Mg0, Al0, Gd0, Sm0) |0 0 |2 EndNote | ||
336 | |a Output Types/Journal article |2 DataCite | ||
336 | |a Journal Article |0 PUB:(DE-HGF)16 |2 PUB:(DE-HGF) | ||
336 | |a article |2 DRIVER | ||
336 | |a JOURNAL_ARTICLE |2 ORCID | ||
981 | |a I:(DE-Juel1)IBI-7-20200312 | ||
920 | |k Molekulare Biophysik; INB-2 |0 I:(DE-Juel1)VDB805 |d 31.12.2008 |g INB |l Molekulare Biophysik |x 0 | ||
981 | |a I:(DE-Juel1)ICS-6-20110106 | ||
981 | |a I:(DE-Juel1)ISB-2-20090406 | ||
990 | |a Stadler, A.M. |0 P:(DE-Juel1)VDB78506 |b 0 |u FZJ | ||
991 | |a Büldt, Georg |0 P:(DE-Juel1)131957 |b 5 |u FZJ | ||
991 | |0 P:(DE-HGF)0 |a Zaccai, G. |b 4 | ||
991 | |0 P:(DE-HGF)0 |a Embs, J.P. |b 3 | ||
991 | |0 P:(DE-HGF)0 |a Artmann, G.M. |b 2 | ||
991 | |0 P:(DE-HGF)0 |a Digel, I. |b 1 |