Description: Metabolic remodeling of the human red blood cell membrane

This title appears in the Scientific Report : 2010

Metabolic remodeling of the human red blood cell membrane

The remarkable deformability of the human red blood cell (RBC) results from the coupled dynamic response of the phospholipid bilayer and the spectrin molecular network. Here we present quantitative connections between spectrin morphology and membrane fluctuations of human RBCs by using dynamic full-...

Personal Name(s):	Park, Y.K.
	Best, C.A. / Auth, T. / Gov, N. S. / Safran, S.A. / Popescu, G. / Suresh, S. / Feld, M.S.
Contributing Institute:	Theorie der Weichen Materie und Biophysik; IAS-2 Theorie der Weichen Materie und Biophysik; IFF-2
Published in:	Proceedings of the National Academy of Sciences of the United States of America, 107 (2010) S. 1289 - 1294
Imprint:	Washington, DC Academy 2010
Physical Description:	1289 - 1294
DOI:	10.1073/pnas.0910785107
PubMed ID:	20080583
Document Type:	Journal Article
Research Program:	BioSoft: Makromolekulare Systeme und biologische Informationsverarbeitung
Series Title:	Proceedings of the National Academy of Sciences of the United States of America 107
Subject (ZB):	Adenosine Triphosphate: metabolism Cell Shape Erythrocyte Membrane: metabolism Erythrocytes: cytology Erythrocytes: metabolism Humans Temperature Adenosine Triphosphate J ATP imaging technique membrane fluctuation RBC spectrin
	Publikationsportal JuSER

Please use the identifier: http://dx.doi.org/10.1073/pnas.0910785107 in citations.

The remarkable deformability of the human red blood cell (RBC) results from the coupled dynamic response of the phospholipid bilayer and the spectrin molecular network. Here we present quantitative connections between spectrin morphology and membrane fluctuations of human RBCs by using dynamic full-field laser interferometry techniques. We present conclusive evidence that the presence of adenosine 5'-triphosphate (ATP) facilitates non-equilibrium dynamic fluctuations in the RBC membrane that are highly correlated with the biconcave shape of RBCs. Spatial analysis of the fluctuations reveals that these non-equilibrium membrane vibrations are enhanced at the scale of spectrin mesh size. Our results indicate that the dynamic remodeling of the coupled membranes powered by ATP results in non-equilibrium membrane fluctuations manifesting from both metabolic and thermal energies and also maintains the biconcave shape of RBCs.