This title appears in the Scientific Report :
2010
Please use the identifier:
http://dx.doi.org/10.1002/mas.20239 in citations.
Bioimaging of metals by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS)
Bioimaging of metals by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS)
The distribution analysis of (essential, beneficial, or toxic) metals (e.g., Cu, Fe, Zn, Pb, and others), metalloids, and non-metals in biological tissues is of key interest in life science. Over the past few years, the development and application of several imaging mass spectrometric techniques has...
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Personal Name(s): | Becker, J. S. |
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Zoriy, M. / Matusch, A. / Wu, B. / Salber, D. / Palm, C. / Becker, J. S. | |
Contributing Institute: |
Zentralabteilung für Chemische Analysen; ZCH Strukturelle und funktionelle Organisation des Gehirns; INM-1 Physik der Medizinischen Bildgebung; INM-4 Molekulare Organisation des Gehirns; INM-2 Abteilung Sicherheit und Strahlenschutz; S |
Published in: | Mass spectrometry reviews (2010) S. 156 - 175 |
Imprint: |
New York, NY [u.a.]
Wiley
2010
|
Physical Description: |
156 - 175 |
DOI: |
10.1002/mas.20239 |
PubMed ID: |
19557838 |
Document Type: |
Journal Article |
Research Program: |
Connectivity and Activity Nukleare Sicherheitsforschung Funktion und Dysfunktion des Nervensystems |
Series Title: |
Mass Spectrometry Reviews
29 |
Subject (ZB): | |
Publikationsportal JuSER |
The distribution analysis of (essential, beneficial, or toxic) metals (e.g., Cu, Fe, Zn, Pb, and others), metalloids, and non-metals in biological tissues is of key interest in life science. Over the past few years, the development and application of several imaging mass spectrometric techniques has been rapidly growing in biology and medicine. Especially, in brain research metalloproteins are in the focus of targeted therapy approaches of neurodegenerative diseases such as Alzheimer's and Parkinson's disease, or stroke, or tumor growth. Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) using double-focusing sector field (LA-ICP-SFMS) or quadrupole-based mass spectrometers (LA-ICP-QMS) has been successfully applied as a powerful imaging (mapping) technique to produce quantitative images of detailed regionally specific element distributions in thin tissue sections of human or rodent brain. Imaging LA-ICP-QMS was also applied to investigate metal distributions in plant and animal sections to study, for example, the uptake and transport of nutrient and toxic elements or environmental contamination. The combination of imaging LA-ICP-MS of metals with proteomic studies using biomolecular mass spectrometry identifies metal-containing proteins and also phosphoproteins. Metal-containing proteins were imaged in a two-dimensional gel after electrophoretic separation of proteins (SDS or Blue Native PAGE). Recent progress in LA-ICP-MS imaging as a stand-alone technique and in combination with MALDI/ESI-MS for selected life science applications is summarized. |