Description: Monomeric Amyloid Beta Peptide in Hexafluoroisopropanol Detected by Small Angle Neutron Scattering

This title appears in the Scientific Report : 2016

Monomeric Amyloid Beta Peptide in Hexafluoroisopropanol Detected by Small Angle Neutron Scattering

Small proteins like amyloid beta (Aβ) monomers are related to neurodegenerative disorders by aggregation to insoluble fibrils. Small angle neutron scattering (SANS) is a nondestructive method to observe the aggregation process in solution. We show that SANS is able to resolve monomers of small molec...

Personal Name(s):	Zhang-Haagen, Bo
	Biehl, Ralf (Corresponding author) / Nagel-Steger, Luitgard / Radulescu, Aurel / Richter, Dieter / Willbold, Dieter (Corresponding author)
Contributing Institute:	Neutronenstreuung; ICS-1 Neutronenstreuung; JCNS-1 JCNS-FRM-II; JCNS-FRM-II Strukturbiochemie; ICS-6
Published in:	PLoS one, 11 (2016) 2, S. e0150267 -
Imprint:	Lawrence, Kan. PLoS 2016
PubMed ID:	26919121
DOI:	10.1371/journal.pone.0150267
Document Type:	Journal Article
Research Program:	Physical Basis of Diseases FRM II / MLZ
Subject (ZB):	Biology > 0 Health and Life > 1 Health and Life > 0
Link:	OpenAccess OpenAccess
	Publikationsportal JuSER

Please use the identifier: http://hdl.handle.net/2128/10163 in citations.
Please use the identifier: http://dx.doi.org/10.1371/journal.pone.0150267 in citations.

Small proteins like amyloid beta (Aβ) monomers are related to neurodegenerative disorders by aggregation to insoluble fibrils. Small angle neutron scattering (SANS) is a nondestructive method to observe the aggregation process in solution. We show that SANS is able to resolve monomers of small molecular weight like Aβ for aggregation studies. We examine Aβ monomers after prolonged storing in d-hexafluoroisopropanol (dHFIP) by using SANS and dynamic light scattering (DLS). We determined the radius of gyration from SANS as 1.0±0.1 nm for Aβ1–40 and 1.6±0.1 nm for Aβ1–42 in agreement with 3D NMR structures in similar solvents suggesting a solvent surface layer with 5% increased density. After initial dissolution in dHFIP Aβ aggregates sediment with a major component of pure monomers showing a hydrodynamic radius of 1.8±0.3 nm for Aβ1–40 and 3.2±0.4 nm for Aβ1–42 including a surface layer of dHFIP solvent molecules.