This title appears in the Scientific Report :
2016
Please use the identifier:
http://dx.doi.org/10.1016/j.tet.2016.08.022 in citations.
Inducing secondary metabolite production by the endophytic fungus Chaetomium sp. through fungal–bacterial co-culture and epigenetic modification
Inducing secondary metabolite production by the endophytic fungus Chaetomium sp. through fungal–bacterial co-culture and epigenetic modification
Co-culturing the fungal endophyte Chaetomium sp. with the bacterium Bacillus subtilis on solid rice medium resulted in an up to 8.3-fold increase in the accumulation of constitutively present metabolites that included a 1:1 mixture of 3- and 4-hydroxybenzoic acid methyl esters (1 and 2, respectively...
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Personal Name(s): | Akone, Sergi Herve |
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Mándi, Attila / Kurtán, Tibor / Hartmann, Rudolf / Lin, Wenhan / Daletos, Georgios / Proksch, Peter (Corresponding author) | |
Contributing Institute: |
Strukturbiochemie; ICS-6 |
Published in: | Tetrahedron, 72 (2016) 41, S. 6340 - 6347 |
Imprint: |
Amsterdam [u.a.]
Elsevier Science
2016
|
DOI: |
10.1016/j.tet.2016.08.022 |
Document Type: |
Journal Article |
Research Program: |
Engineering Cell Function |
Publikationsportal JuSER |
Co-culturing the fungal endophyte Chaetomium sp. with the bacterium Bacillus subtilis on solid rice medium resulted in an up to 8.3-fold increase in the accumulation of constitutively present metabolites that included a 1:1 mixture of 3- and 4-hydroxybenzoic acid methyl esters (1 and 2, respectively), and the polyketides acremonisol A (3), SB236050 (4), and SB238569 (5). In addition, seven compounds including isosulochrin (6), protocatechuic acid methyl ester (7), as well as five new natural products (8e12) were detected in the co-cultures, but not in axenic fungal cultures. Treatment of Chaetomium sp. with the epigenetic modifier suberoylanilide hydroxamic acid or 5-azacytidine resulted in an enhanced accumulation of 6, which was likewise detected during co-culture. Compound 5 showed strong cytotoxicity against the mouse lymphoma L5178Y cell line with an IC50 value of 1 mM, as well as weak antibacterial activity against B. subtilis with an MIC value of 53 mM. |