This title appears in the Scientific Report :
2016
Please use the identifier:
http://dx.doi.org/10.1002/anie.201603205 in citations.
Selective Protein Hyperpolarization in Cell Lysates Using Targeted Dynamic Nuclear Polarization
Selective Protein Hyperpolarization in Cell Lysates Using Targeted Dynamic Nuclear Polarization
Nuclear magnetic resonance (NMR) spectroscopy has the intrinsic capabilities to investigate proteins in native environments. In general, however, NMR relies on non-natural protein purity and concentration to increase the desired signal over the background. We here report on the efficient and specifi...
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Personal Name(s): | Viennet, Thibault |
---|---|
Viegas, Aldino / Kuepper, Arne / Arens, Sabine / Gelev, Vladimir / Petrov, Ognyan / Grossmann, Tom N. / Heise, Henrike / Etzkorn, Manuel (Corresponding author) | |
Contributing Institute: |
Strukturbiochemie; ICS-6 |
Published in: | Angewandte Chemie / International edition, 55 (2016) 36, S. 10746 - 10750 |
Imprint: |
Weinheim
Wiley-VCH
2016
|
DOI: |
10.1002/anie.201603205 |
PubMed ID: |
27351143 |
Document Type: |
Journal Article |
Research Program: |
Functional Macromolecules and Complexes |
Publikationsportal JuSER |
Nuclear magnetic resonance (NMR) spectroscopy has the intrinsic capabilities to investigate proteins in native environments. In general, however, NMR relies on non-natural protein purity and concentration to increase the desired signal over the background. We here report on the efficient and specific hyperpolarization of low amounts of a target protein in a large isotope-labeled background by combining dynamic nuclear polarization (DNP) and the selectivity of protein interactions. Using a biradical-labeled ligand, we were able to direct the hyperpolarization to the protein of interest, maintaining comparable signal enhancement with about 400-fold less radicals than conventionally used. We could selectively filter out our target protein directly from crude cell lysate obtained from only 8 mL of fully isotope-enriched cell culture. Our approach offers effective means to study proteins with atomic resolution in increasingly native concentrations and environments. |