This title appears in the Scientific Report :
2016
Please use the identifier:
http://dx.doi.org/10.1016/j.ymben.2016.10.007 in citations.
Production of 2-methyl-1-butanol and 3-methyl-1-butanol in engineered Corynebacterium glutamicum
Production of 2-methyl-1-butanol and 3-methyl-1-butanol in engineered Corynebacterium glutamicum
The pentanol isomers 2-methyl-1-butanol and 3-methyl-1-butanol represent commercially interesting alcohols due to their potential application as biofuels. For a sustainable microbial production of these compounds, Corynebacterium glutamicum was engineered for producing 2-methyl-1-butanol and 3-methy...
| Personal Name(s): | Vogt, Michael |
|---|---|
| Brüsseler, Christian / Ooyen, Jan van / Bott, Michael / Marienhagen, Jan (Corresponding author) | |
| Contributing Institute: |
Biotechnologie; IBG-1 |
| Published in: | Metabolic engineering, 38 (2016) S. 436–445 |
| Imprint: |
Orlando, Fla.
Academic Press
2016
|
| DOI: |
10.1016/j.ymben.2016.10.007 |
| Document Type: |
Journal Article |
| Research Program: |
Biotechnology |
| Publikationsportal JuSER |
|
The pentanol isomers 2-methyl-1-butanol and 3-methyl-1-butanol represent commercially interesting alcohols due to their potential application as biofuels. For a sustainable microbial production of these compounds, Corynebacterium glutamicum was engineered for producing 2-methyl-1-butanol and 3-methyl-1-butanol via the Ehrlich pathway from 2-keto-3-methylvalerate and 2-ketoisocaproate, respectively. In addition to an already available 2-ketoisocaproate producer, a 2-keto-3-methylvalerate accumulating C. glutamicum strain was also constructed. For this purpose, we reduced the activity of the branched-chain amino acid transaminase in an available C. glutamicuml-isoleucine producer (K2P55) via a start codon exchange in the ilvE gene enabling accumulation of up to 3.67 g/l 2-keto-3-methylvalerate. Subsequently, nine strains expressing different gene combinations for three 2-keto acid decarboxylases and three alcohol dehydrogenases were constructed and characterized. The best strains accumulated 0.37 g/l 2-methyl-1-butanol and 2.76 g/l 3-methyl-1-butanol in defined medium within 48 h under oxygen deprivation conditions, making these strains ideal candidates for additional strain and process optimization. |