This title appears in the Scientific Report :
2016
Please use the identifier:
http://dx.doi.org/10.1021/jacs.6b05475 in citations.
Please use the identifier: http://hdl.handle.net/2128/12752 in citations.
A Self-Activated Mechanism for Nucleic Acid Polymerization Catalyzed by DNA/RNA Polymerases
A Self-Activated Mechanism for Nucleic Acid Polymerization Catalyzed by DNA/RNA Polymerases
The enzymatic polymerization of DNA and RNA is the basis for genetic inheritance for all living organisms. It is catalyzed by the DNA/RNA polymerase (Pol) superfamily. Here, bioinformatics analysis reveals that the incoming nucleotide substrate always forms an H-bond between its 3′-OH and β-phosphat...
Saved in:
Personal Name(s): | Genna, Vito |
---|---|
Vidossich, Pietro / Ippoliti, Emiliano / Carloni, Paolo (Corresponding author) / Vivo, Marco De (Corresponding author) | |
Contributing Institute: |
Computational Biomedicine; INM-9 Computational Biomedicine; IAS-5 |
Published in: | Journal of the American Chemical Society, 138 (2016) 44, S. 14592 - 14598 |
Imprint: |
Washington, DC
American Chemical Society
2016
|
PubMed ID: |
27530537 |
DOI: |
10.1021/jacs.6b05475 |
Document Type: |
Journal Article |
Research Program: |
Addenda |
Link: |
OpenAccess OpenAccess |
Publikationsportal JuSER |
Please use the identifier: http://hdl.handle.net/2128/12752 in citations.
LEADER | 05872nam a2200889 a 4500 | ||
---|---|---|---|
001 | 820729 | ||
005 | 20210129224604.0 | ||
024 | 7 | |a 10.1021/jacs.6b05475 |2 doi | |
024 | 7 | |a 0002-7863 |2 ISSN | |
024 | 7 | |a 1520-5126 |2 ISSN | |
024 | 7 | |a 2128/12752 |2 Handle | |
024 | 7 | |a WOS:000387625300018 |2 WOS | |
024 | 7 | |a altmetric:10576108 |2 altmetric | |
024 | 7 | |a pmid:27530537 |2 pmid | |
037 | |a FZJ-2016-05997 | ||
082 | |a 540 | ||
100 | 1 | |a Genna, Vito |0 P:(DE-HGF)0 |b 0 | |
245 | |a A Self-Activated Mechanism for Nucleic Acid Polymerization Catalyzed by DNA/RNA Polymerases | ||
260 | |a Washington, DC |c 2016 |b American Chemical Society | ||
520 | |a The enzymatic polymerization of DNA and RNA is the basis for genetic inheritance for all living organisms. It is catalyzed by the DNA/RNA polymerase (Pol) superfamily. Here, bioinformatics analysis reveals that the incoming nucleotide substrate always forms an H-bond between its 3′-OH and β-phosphate moieties upon formation of the Michaelis complex. This previously unrecognized H-bond implies a novel self-activated mechanism (SAM), which synergistically connects the in situ nucleophile formation with subsequent nucleotide addition and, importantly, nucleic acid translocation. Thus, SAM allows an elegant and efficient closed-loop sequence of chemical and physical steps for Pol catalysis. This is markedly different from previous mechanistic hypotheses. Our proposed mechanism is corroborated via ab initio QM/MM simulations on a specific Pol, the human DNA polymerase-η, an enzyme involved in repairing damaged DNA. The structural conservation of DNA and RNA Pols supports the possible extension of SAM to Pol enzymes from the three domains of life. | ||
588 | |a Dataset connected to CrossRef | ||
700 | 1 | |a Vidossich, Pietro |0 P:(DE-HGF)0 |b 1 | |
700 | 1 | |a Ippoliti, Emiliano |0 P:(DE-Juel1)146009 |b 2 |u fzj | |
700 | 1 | |a Carloni, Paolo |0 P:(DE-Juel1)145614 |b 3 |e Corresponding author | |
700 | 1 | |a Vivo, Marco De |0 P:(DE-HGF)0 |b 4 |e Corresponding author | |
773 | |a 10.1021/jacs.6b05475 |g Vol. 138, no. 44, p. 14592 - 14598 |0 PERI:(DE-600)1472210-0 |n 44 |p 14592 - 14598 |t Journal of the American Chemical Society |v 138 |y 2016 |x 1520-5126 | ||
856 | 4 | |y OpenAccess |u http://juser.fz-juelich.de/record/820729/files/jacs.6b05475.pdf | |
856 | 4 | |y OpenAccess |x icon |u http://juser.fz-juelich.de/record/820729/files/jacs.6b05475.gif?subformat=icon | |
856 | 4 | |y OpenAccess |x icon-1440 |u http://juser.fz-juelich.de/record/820729/files/jacs.6b05475.jpg?subformat=icon-1440 | |
856 | 4 | |y OpenAccess |x icon-180 |u http://juser.fz-juelich.de/record/820729/files/jacs.6b05475.jpg?subformat=icon-180 | |
856 | 4 | |y OpenAccess |x icon-640 |u http://juser.fz-juelich.de/record/820729/files/jacs.6b05475.jpg?subformat=icon-640 | |
856 | 4 | |y OpenAccess |x pdfa |u http://juser.fz-juelich.de/record/820729/files/jacs.6b05475.pdf?subformat=pdfa | |
909 | C | O | |o oai:juser.fz-juelich.de:820729 |p openaire |p open_access |p driver |p VDB |p dnbdelivery |
910 | 1 | |a Forschungszentrum Jülich |0 I:(DE-588b)5008462-8 |k FZJ |b 2 |6 P:(DE-Juel1)146009 | |
910 | 1 | |a Forschungszentrum Jülich |0 I:(DE-588b)5008462-8 |k FZJ |b 3 |6 P:(DE-Juel1)145614 | |
910 | 1 | |a Forschungszentrum Jülich |0 I:(DE-588b)5008462-8 |k FZJ |b 4 |6 P:(DE-HGF)0 | |
913 | 1 | |a DE-HGF |b Programmungebundene Forschung |l ohne Programm |1 G:(DE-HGF)POF3-890 |0 G:(DE-HGF)POF3-899 |2 G:(DE-HGF)POF3-800 |v ohne Topic |x 0 |4 G:(DE-HGF)POF |3 G:(DE-HGF)POF3 | |
914 | 1 | |y 2016 | |
915 | |a DBCoverage |0 StatID:(DE-HGF)0200 |2 StatID |b SCOPUS | ||
915 | |a DBCoverage |0 StatID:(DE-HGF)1030 |2 StatID |b Current Contents - Life Sciences | ||
915 | |a DBCoverage |0 StatID:(DE-HGF)0600 |2 StatID |b Ebsco Academic Search | ||
915 | |a Free to read |0 LIC:(DE-HGF)PublisherOA |2 HGFVOC | ||
915 | |a JCR |0 StatID:(DE-HGF)0100 |2 StatID |b J AM CHEM SOC : 2015 | ||
915 | |a IF >= 10 |0 StatID:(DE-HGF)9910 |2 StatID |b J AM CHEM SOC : 2015 | ||
915 | |a DBCoverage |0 StatID:(DE-HGF)0150 |2 StatID |b Web of Science Core Collection | ||
915 | |a WoS |0 StatID:(DE-HGF)0110 |2 StatID |b Science Citation Index | ||
915 | |a WoS |0 StatID:(DE-HGF)0111 |2 StatID |b Science Citation Index Expanded | ||
915 | |a OpenAccess |0 StatID:(DE-HGF)0510 |2 StatID | ||
915 | |a Peer Review |0 StatID:(DE-HGF)0030 |2 StatID |b ASC | ||
915 | |a DBCoverage |0 StatID:(DE-HGF)1150 |2 StatID |b Current Contents - Physical, Chemical and Earth Sciences | ||
915 | |a DBCoverage |0 StatID:(DE-HGF)0310 |2 StatID |b NCBI Molecular Biology Database | ||
915 | |a DBCoverage |0 StatID:(DE-HGF)1050 |2 StatID |b BIOSIS Previews | ||
915 | |a DBCoverage |0 StatID:(DE-HGF)0300 |2 StatID |b Medline | ||
915 | |a Nationallizenz |0 StatID:(DE-HGF)0420 |2 StatID | ||
915 | |a DBCoverage |0 StatID:(DE-HGF)0199 |2 StatID |b Thomson Reuters Master Journal List | ||
980 | |a journal | ||
980 | |a VDB | ||
980 | |a UNRESTRICTED | ||
980 | |a I:(DE-Juel1)IAS-5-20120330 | ||
980 | |a I:(DE-Juel1)INM-9-20140121 | ||
980 | 1 | |a FullTexts | |
536 | |a Addenda |0 G:(DE-HGF)POF3-899 |c POF3-899 |f POF III |x 0 | ||
336 | |a ARTICLE |2 BibTeX | ||
336 | |a Journal Article |b journal |m journal |0 PUB:(DE-HGF)16 |s 1478846521_32300 |2 PUB:(DE-HGF) | ||
336 | |a Output Types/Journal article |2 DataCite | ||
336 | |a article |2 DRIVER | ||
336 | |a Nanopartikel unedler Metalle (Mg0, Al0, Gd0, Sm0) |0 0 |2 EndNote | ||
336 | |a JOURNAL_ARTICLE |2 ORCID | ||
981 | |a I:(DE-Juel1)INM-9-20140121 | ||
920 | |l yes | ||
920 | |k Computational Biomedicine; INM-9 |0 I:(DE-Juel1)INM-9-20140121 |l Computational Biomedicine |x 1 | ||
920 | |k Computational Biomedicine; IAS-5 |0 I:(DE-Juel1)IAS-5-20120330 |l Computational Biomedicine |x 0 | ||
990 | |a Genna, Vito |0 P:(DE-HGF)0 |b 0 | ||
991 | |a Vivo, Marco De |0 P:(DE-HGF)0 |b 4 |e Corresponding author | ||
991 | |a Carloni, Paolo |0 P:(DE-Juel1)145614 |b 3 |e Corresponding author | ||
991 | |a Ippoliti, Emiliano |0 P:(DE-Juel1)146009 |b 2 |u fzj | ||
991 | |a Vidossich, Pietro |0 P:(DE-HGF)0 |b 1 |