On the use of $^{14}$C and $^{32}$P in locating genetically caused defects in photosynthesis of some plastid mutants
On the use of $^{14}$C and $^{32}$P in locating genetically caused defects in photosynthesis of some plastid mutants
Today it is well known that all reactions of cellular metabolic physiology are regulated by the regulatory effects of genes. This dependence of biochemical reactions on genes can be investigated under favourable circumstances with the help of certain suitable mutants. The place of activity of certai...
| Personal Name(s): | Hallier, U. W. (Corresponding author) |
|---|---|
| Contributing Institute: |
Publikationen vor 2000; PRE-2000; Retrocat |
| Imprint: |
Jülich
Kernforschungsanlage Jülich, Verlag
1967
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| Physical Description: |
11 p. |
| Document Type: |
Report Book |
| Research Program: |
ohne Topic |
| Series Title: |
Berichte der Kernforschungsanlage Jülich
489 |
| Link: |
OpenAccess OpenAccess |
| Publikationsportal JuSER |
|
Today it is well known that all reactions of cellular metabolic physiology are regulated by the regulatory effects of genes. This dependence of biochemical reactions on genes can be investigated under favourable circumstances with the help of certain suitable mutants. The place of activity of certain genes can be localized by biochemical methods as weil as by using radioisotopes. The extensive complex of reactions of which photosynthesis is composed was checked on some plastidemutants of Oenothera suaveolens. These planes are lethal mutants which, from their development and appearance, are assumed to starve because of insufficient photosynthetic actiility. ln searching for such a possible genetically caused block in the mechanism of photosynthesis, the respiratory and photosynthetic effect was checked and the intensity of assimilation was tested by the use of $^{14}CO_{2}$. The carbon cycle was divided into partial processes checked in vitro one by one; then the light-dependent reactions of photosynthesis, leading to formation of ATP and NADPH, were investigated as well as, by means of $^{32}$p, the ability of photophosphorylation. As the results point out, the defect of the mutants leading to lethality must be assumed within the chain of electron transport. |